Effect and mechanism of ellagic acid on muscle function improvement in mice

LU Yuting; LI Jun; ZHOU Xianjie; LV Yuxia; LU Feixiang; LIU Qingchun

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Medical Journal of the Chinese People Armed Police Forces ›› 2020, Vol. 31 ›› Issue (4) : 324-327.

ORIGINAL ARTICLES

Effect and mechanism of ellagic acid on muscle function improvement in mice

LU Yuting¹, LI Jun², ZHOU Xianjie¹, LV Yuxia¹, LU Feixiang³, LIU Qingchun¹

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Abstract

Objective To observe the effect of ellagic acid(EA) on muscle function in normal mice.Methods ICR male mice were randomly divided into the control group and EA treatment group, with 8 mice in each. The EA treatment group was given 10 mg/100 g weight of EA per day while the control group was given an equal volume of solvent once daily for 21 days. The Echo MRITM was used to determine the body composition of the mice, and the grip tester was used to measure the grip strength of the mice. The times of drops were measured by the rotary fatigue meter,and the cross-sectional area of gastrocnemius muscle fibers was calculated by HE staining.qRT-PCR was used to analyze MuRF1 and Atrogin-1 gene expressions in the ubiquitin protease pathway.Results After EA treatment,there was no statistically significant difference in body composition between the two groups. Compared with the control group, the mice in the EA treatment group increased their grip strength and cross-sectional area of muscle fibers,but reduced the times of drops and the mRNA expression levels of MuRF1 and Atrogin-1.The difference was statistically significant (P<0.05).Conclusions Ellagic acid may inhibit the degradation of proteins, enhance the muscle function of mice, and improve exercise endurance in mice. It is expected to be a plant compound that improves muscle strength and exercise function.

Key words

ellagic acid / body composition / muscle function / MuRF1 / Atrogin-1

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LU Yuting, LI Jun, ZHOU Xianjie, LV Yuxia, LU Feixiang, LIU Qingchun. Effect and mechanism of ellagic acid on muscle function improvement in mice[J]. Medical Journal of the Chinese People Armed Police Forces. 2020, 31(4): 324-327

References

[1] Yan B,Liu Y,Shi A,et al. Investigation of the antifatigue effects of korean ginseng on professional athletes by gas chromatography- time- of- flight- mass spectrometry- based metabolomics [J]. J AOAC Int,2018,101(3):701-707.
[2] Sousa A S,Guerra R S,Fonseca I,et al. Financial impact of sarcopenia on hospitalization costs[J]. Eur J Clin Nutr,2016,70(9):1046-1051.
[3] Bjorklund G,Dadar M,Pen J J,et al. Chronic fatigue syndrome (CFS): suggestions for a nutritional treatment in the therapeutic approach[J]. Biomed Pharmacother,2019,109(1):1000-1007.
[4] Gonzalez-barrio R,Truchado P,Ito H,et al. UV and MS identification of urolithins and nasutins,the bioavailable metabolites of ellagitannins and ellagic acid in different mammals[J]. J Agric Food Chem,2011,59(4):1152-1162.
[5] Cortes-martin A,Garcia-villalba R,Gonzalez-sarrias A,et al. The gut microbiota urolithin metabotypes revisited:the human metabolism of ellagic acid is mainly determined by aging[J]. Food Funct,2018,9(8):4100-4106.
[6] Larrosa M,Garcia-conesa M T,Espin J C,et al. Ellagitannins,ellagic acid and vascular health[J]. Mol Aspects Med,2010,31(6):513-539.
[7] Kawabata K,Yoshioka Y,Terao J. Role of intestinal microbiota in the bioavailability and physiological functions of dietary polyphenols[J]. Molecules,2019,24(2):370.
[8] Landete J M. Ellagitannins,ellagic acid and their derived metabolites: a review about source, metabolism, functions and health[J]. Food Research International,2011,44(5):1150-1160.
[9] Ceci C, Tentori L, Atzori M G, et al. Ellagic acid inhibits bladder cancer invasiveness and in vivo tumor growth[J]. Nutrients,2016,8(11):744.
[10] Li Z, Henning S M, Lee R P, et al. Pomegranate extract induces ellagitannin metabolite formation and changes stool microbiota in healthy volunteers[J]. Food Funct, 2015,6(8):2487-2495.
[11] Chen B, Tuuli M G, Longtine M S, et al. Pomegranate juice and punicalagin attenuate oxidative stress and apoptosis in human placenta and in human placental trophoblasts[J]. Am J Physiol Endocrinol Metab,2012,302(9):E1142-1152.
[12] Alamdari N,Aversa Z,Castillero E,et al. Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1-dependent mechanism[J]. Biochem Biophys Res Commun,2012,417(1):528-533.
[13] Francaux M,Deldicque L. Using polyphenol derivatives to prevent muscle wasting[J]. Curr Opin Clin Nutr Metab Care,2018,21(3):159-163.
[14] Trombold J R,Reinfeld A S,Casler J R,et al. The effect of pomegranate juice supplementation on strength and soreness after eccentric exercise[J]. J Strength Cond Res,2011,25(7):1782-1788.
[15] Bodine S C,Latres E,Baumhueter S,et al. Identification of ubiquitin ligases required for skeletal muscle atrophy[J]. Science,2001,294(5547):1704-1708.
[16] Ito H, Iguchi A, Hatano T. Identification of urinary and intestinal bacterial metabolites of ellagitannin geraniin in rats[J]. J Agric Food Chem. 2008,56(2):393-400.
[17] 王丛丛.尿石素B对地塞米松诱导小鼠肌萎缩的改善作用及机制[J]. 中国老年学杂志, 2018,8(38):1963-1966.
[18] Bennett B T,Mohamed J S,Alway S E. Effects of resveratrol on the recovery of muscle mass following disuse in the plantaris muscle of aged rats[J]. PLoS One,2013,8(12): 83518.
[19] Barbalho E R,Gonzalez M C,Bielemann R M,et al. Is skeletal muscle radiodensity able to indicate physical function impairment in older adults with gastrointestinal cancer[J]. Exp Gerontol,2019,125(1):110688-110688.
[20] Saifetiarova J,Liu X,Taylor A M,et al. Axonal domain disorganization in Caspr1 and Caspr2 mutant myelinated axons affects neuromuscular junction integrity, leading to muscle atrophy[J]. J Neurosci Res,2017,95(7):1373-1390.
[21] Zheng Y,Zhang W C,Wu Z Y,et al. Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice[J]. J Sci Food Agric,2019,99(3):1405-1412.
[22] Schiaffino S,Reggiani C. Fiber types in mammalian skeletal muscles[J]. Physiol Rev, 2011,91(4):1447-1531.
[23] Jackman R W,Kandarian S C. The molecular basis of skeletal muscle atrophy[J]. Am J Physiol Cell Physiol,2004,287(4):834-843.