Human umbilical cord mesenchymal stem cells derived exosomes promote proliferation and differentiation of osteoblast progenitor cells

WANG Li; DUAN Cuimi; YUAN Fangfang; WANG Yan; GUO Ximin; GUO Hongyan

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Medical Journal of the Chinese People Armed Police Forces ›› 2020, Vol. 31 ›› Issue (4) : 335-339.

Human umbilical cord mesenchymal stem cells derived exosomes promote proliferation and differentiation of osteoblast progenitor cells

WANG Li^1,2, DUAN Cuimi³, YUAN Fangfang¹, WANG Yan³, GUO Ximin³, GUO Hongyan^1,2

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Abstract

Objective To extract human umbilical cord mesenchymal stem cells (hucMSCs) derived exosomes (hucMSC-exos) and observe their effects on mouse osteoblast progenitor cells (mOPCs).Methods HucMSCs were isolated and cultured in vitro. Exosomes were collected by ultracentrifugation from supernatants. After treatment with hucMSCs-exos at different concentrations, the proliferation of mOPCs was detected by CCK8 assay. Alkaline phosphatase activity (ALP) analysis and alizarin red staining were used to detect the effects of different concentrations of hucMSC-exo on the mineralization process of mOPCs.Results hucMSCs were derived that exhibited spindle-like morphology, vortex-shaped adherent growth and the potential for multidirectional induction of differentiation. HucMSC-exos were isolated that had spherical structures and a size of around 80nm. The results of Western blot analysis showed that hucMSC-exos expressed the exosomal markers CD9 and CD63. The Results of CCK8 showed that 5 μg/ml and 10 μg/ml hucMSC-exos could significantly promote the mOPC proliferation in vitro (P<0.05). The Results of alkaline phosphatase and alizarin red staining revealed the 5 μg/ml and 10 μg/ml hucMSC-exo group could significantly increase the alkaline phosphatase activity and calcified nodule formation of mOPCs. The role of 10 μg/ml exosomes was stronger than that of 5 μg/ml (P<0.05).Conclusions hucMSCs and hucMSC-exo have been isolated. hucMSC-exos can effectively promote the proliferation and osteogenic differentiation of mOPCs in a dose-dependent manner.

Key words

human umbilical cord mesenchymal stem cell / exosome / osteoblast progenitor cell / proliferation / osteogenic differentiation

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WANG Li, DUAN Cuimi, YUAN Fangfang, WANG Yan, GUO Ximin, GUO Hongyan. Human umbilical cord mesenchymal stem cells derived exosomes promote proliferation and differentiation of osteoblast progenitor cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2020, 31(4): 335-339

References

[1] Rezaie J, Ajezi S, Avci B, et al. Exosomes and their application in biomedical field: difficulties and advantages[J]. Mol Neurobiol, 2017, 55(3): 1-22.
[2] 陈燕,姜胜军,彭友俭. 骨髓间充质干细胞来源的外泌体对成骨细胞增殖和分化的影响[J]. 口腔医学研究, 2019, 35(4): 94-97.
[3] Ding D C, Shyu W C, Lin S Z. Mesenchymal stem cells[J]. Cell Transplant, 2011, 20(1): 5-14.
[4] Ding M, Shen Y, Wang P, et al. Exosomes isolated from human umbilical cord mesenchymal stem cells alleviate neuroinflammation and reduce Amyloid-Beta deposition by modulating microglial activation in Alzheimer’s disease[J]. Neurochem Res, 2018, 43(11): 2165-2177.
[5] 杨卉馨,黄小会,董晓惠,等. 高糖环境对小鼠成骨前体细胞及脂肪干细胞成骨分化的影响[J]. 解放军医学院学报, 2019 (5): 449-453.
[6] Koh S H, Kim K S, Choi M R, et al. Implantation of human umbilical cord-derived mesenchymal stem cells as a neuroprotective therapy for ischemic stroke in rats[J]. Brain Res, 2008, 1229(10): 233-248.
[7] Zheng Y T, Hao Z C,Wang P F, et al. Exosomes from human umbilical cord mesenchymal stem cells enhance fracture healing through HIF-1α-mediated promotion of angiogenesis in a rat model of stabilized fracture[J]. Cell Proliferat, 2019, 52(2): e12570.
[8] Feng Y, Lu S H, Wang X, et al. Biological characteristics of exosomes secreted by human bone marrow mesenchymal stem cells[J]. Exp Hematol, 2014, 22(3): 595-599.
[9] Quarto R, Mastrogiacomo M, Cancedda R, et al. Repair of large bone defects with the use of autologous bone marrow stromal cells[J]. New Engl J Med, 2001, 344(5): 385-386.
[10] Wei C C, Lin A B, Hung S C. Mesenchymal stem cells in regenerative medicine for musculoskeletal diseases: bench, bedside, and industry[J]. Cell Transplant, 2014, 23(4-5): 505-512.
[11] Wang K W Z J. Enhanced cardioprotection by human endometrium mesenchymal stem cells driven by exosomal microRNA-21[J]. Stem Cell Transl Med, 2017, 6(1); 209-222.
[12] Liang X, Zhang Y, Ding Y, et al. Paracrine mechanisms of mesenchymal stem cell-based therapy: current status and perspectives[J]. Cell Transplant, 2013, 23(9): 1045-1059.
[13] Raghuvaran N, Chun-Chieh H, Sriram R. Hijacking the cellular mail: exosome mediated differentiation of mesenchymal stem cells[J]. Stem Cells Int, 2016, 2016: 1-11.
[14] Qin Y, Wang L, Gao Z, et al. Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo[J]. Sci Rep-UK, 2016, 6: 61-68.
[15] Todeschi M R, El Backly R, Capelli C, et al. Transplanted umbilical cord mesenchymal stem cells modify the in vivo microenvironment enhancing angiogenesis and leading to bone regeneration[J]. Stem Cells Dev, 2015, 24(13): 1570-1581.
[16] Bhagirath D, Yang T, Sekhon K, et al. Abstract 5448: an exosomal biomarker for prostate cancer[J]. Cancer Res, 2017, 77(13): 5448.
[17] Higuchi A, Shen P, Zhao J, et al. Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light[J]. Tissue Eng PT A, 2017,17(21-22): 2593-2602.
[18] Donald, G, Phinney, et al. Concise review: MSC-derived exosomes for cell-free therapy[J]. Stem Cells, 2017, 35(4): 851-858.
[19] Lv C, Yang T. Effective enrichment of urinary exosomes by polyethylene glycol for RNA detection[J]. Biomed Res-India, 2018, 29(8): 1657-1663.
[20] Dang X, Zeng X. Targeted therapeutic delivery using engineered exosomes and its applications in cardiovascular diseases[J]. Gene, 2016, 575(2): 377-384.