Effects of irisin on adipogenesis of bone-marrow mesenchymal stem cells from rabbits

ZHAI Yu; DONG Keming; GUO Hongyan; LIU Cui; XU Hongmei; SHEN Yechun; LI Jinru; MEI Wen

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Medical Journal of the Chinese People Armed Police Forces ›› 2020, Vol. 31 ›› Issue (6) : 512-515.

Effects of irisin on adipogenesis of bone-marrow mesenchymal stem cells from rabbits

ZHAI Yu¹, DONG Keming¹, GUO Hongyan¹, LIU Cui¹, XU Hongmei¹, SHEN Yechun², LI Jinru³, MEI Wen⁴

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Abstract

Objective To investigate the effects of irisin on adipocyte differentiation of BMSCs from rabbits and related mechanisms. Methods BMSCs of rabbits were extracted and proliferation capacity was detected. After BMSCs were cultured in adipogenesis medium supplemented with or without irisin, qPCR was used to evaluate the expression levels of PPARγ and C/EBPα, while oil red O staining was used to investigate fat accumulation. The signaling protein level of Wnt10a was detected by Western blot. Results Proliferation capacity of BMSCs was hardly affected by irisin. Irisin could significantly downregulate mRNA expressions of PPARγ [(0.20±0.06) vs (1.00±0.10) 7 days after adipogenesis induction and(0.61±0.06) vs (1.00±0.15) 14 days after adipogenesis induction]and C/EBPα [(0.40±0.06) vs (1.00±0.12) 7 days after adipogenesis induction and (0.70±0.09) vs (1.00±0.18) 14 days after adipogenesis induction], while attenuating fat accumulation [(0.45±0.05) vs (0.95±0.10),P<0.05]. Moreover, Wnt10a protein levels were increased by irisin intervention[(5.01±0.78) vs (1.00±0.25),P<0.05]. Conclusions Irisin can inhibit adipocyte differentiation of BMSCs from rabbits, and this effect is related to activation of Wnt signaling pathway.

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irisin / adipogenesis / bone-marrow mesenchymal stem cells

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ZHAI Yu, DONG Keming, GUO Hongyan, LIU Cui, XU Hongmei, SHEN Yechun, LI Jinru, MEI Wen. Effects of irisin on adipogenesis of bone-marrow mesenchymal stem cells from rabbits[J]. Medical Journal of the Chinese People Armed Police Forces. 2020, 31(6): 512-515

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