Simultaneous determination of Notoginsenoside R1 and ginsenoside Rf in Shenlong Tongluo pills by HPLC

YANG Yuanyuan; LUAN Tao; SUN Yukun; GUO Zhaogang

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Medical Journal of the Chinese People Armed Police Forces ›› 2021, Vol. 32 ›› Issue (1) : 15-18.

Simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills by HPLC

YANG Yuanyuan¹, LUAN Tao², SUN Yukun², GUO Zhaogang²

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Abstract

Objective To establish an HPLC method for simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills.Methods High performance liquid chromatography was used.Hypersil Hypersil BDS C₁₈(5 μm,4 mm×250 mm) column was selected with acetonitrile-water as the mobile phase for gradient elution.The flow rate was 1.0 ml/min,detection wavelength was 203 nm and the column temperature was set at 35 ℃.Results The linear ranges of Notoginsenoside R₁ and ginsenoside Rf were 0.198-9.535 μg (r=0.9993) and 0.957-38.392 μg(r=0.9997) respectively.The average recoveries were 99.10％(RSD=1.57％,n=6) and 98.76％(RSD=2.13％,n=6)respectively.Conclusions This HPLC method was accurate,reliable and reproducible,which can contribute to formulating the quality standard of Shenlong Tongluo pills.

Key words

Shenlong Tongluo pills / Notoginsenoside R₁ / ginsenoside Rf / HPLC

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YANG Yuanyuan, LUAN Tao, SUN Yukun, GUO Zhaogang. Simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills by HPLC[J]. Medical Journal of the Chinese People Armed Police Forces. 2021, 32(1): 15-18

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