Influence of 1,25(OH)2D3 on proteoglycan 4 gene expression and secretion by rabbit synovial membrane

WANG Zhiqiang; GUANG Chaoling; ZHU Hui; LI Xianhui

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Medical Journal of the Chinese People Armed Police Forces ›› 2021, Vol. 32 ›› Issue (1) : 42-45.

Influence of 1,25(OH)₂D₃ on proteoglycan 4 gene expression and secretion by rabbit synovial membrane

WANG Zhiqiang¹, GUANG Chaoling², ZHU Hui³, LI Xianhui²

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Abstract

Objective To investigate the effects of 1,25(OH)₂D₃ (the active form of vitamin D) on proteoglycan 4 (PRG4) gene expression and secretion by rabbit synovial membrane in order to find an effective scheme for protecting synovial joints and preventing osteoarthritis.Methods The synovial membrane from New Zealand rabbits was cultured.The synovial membrane was treated with 10 nM of 1,25(OH)₂D₃ for different lengths of time and the PRG4 gene expression was evaluated using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR).PRG4 concentrations in the synovial membrane culture supernatant were measured using sandwich enzyme linked immunosorbent assay (ELISA).The optimal time point was determined for the subsequent experiments.After the synovial membrane was treated with different concentrations of 1,25(OH)₂D₃,the effect of 1,25(OH)₂D₃ on PRG4 gene expression and secretion was examined.Results Real-time PCR showed that PRG4 mRNA was up-regulated 6 h after 1,25(OH)₂D₃ treatment,and that PRG4 mRNA expression peaked at 24 h.ELISA results showed that PRG4 protein content in the synovial medium began to increase significantly 12 h after 1,25(OH)₂D₃ treatment,and reached the peak at 48 h.Real-time PCR analysis showed that 1,25(OH)₂D₃ promoted the expression of PRG4 gene in the joint synovial membrane within the range of 0.1-100 nM.The expression of PRG4 gene was increased gradually with 1,25(OH)₂D₃ concentrations.ELISA analysis showed that 1,25(OH)₂D₃ promoted the synovial secretion of PRG4 within the range of 0.1-100 nM.The synovial secretion of PRG4 gradually increased along with 1,25(OH)₂D₃ concentrations.Conclusions Our study has demonstrated that 1,25-(OH)₂D₃ can promote the PRG4 gene expression and secretion of synovial membrane.

Key words

1,25-(OH)₂D₃ / synovial membrane / proteoglycan 4

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WANG Zhiqiang, GUANG Chaoling, ZHU Hui, LI Xianhui. Influence of 1,25(OH)₂D₃ on proteoglycan 4 gene expression and secretion by rabbit synovial membrane[J]. Medical Journal of the Chinese People Armed Police Forces. 2021, 32(1): 42-45

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