Ring finger protein187 promotes the malignancy of glioma cells through pyrolytic pathway

DONG Junqiang; PEI Meijuan; HUANG Shengxuan; LI Xuewen; TENG Chuan

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Medical Journal of the Chinese People Armed Police Forces ›› 2021, Vol. 32 ›› Issue (12) : 1060-1064.

ORIGINAL ARTICLES

Ring finger protein187 promotes the malignancy of glioma cells through pyrolytic pathway

DONG Junqiang¹, PEI Meijuan², HUANG Shengxuan³, LI Xuewen⁴, TENG Chuan⁵

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Abstract

Objective To investigate the effect of RNF187 overexpression on human glioma cell U87 and the possible mechanism. Methods Glioma cell U87 was cultured in vitro and transfected with overexpressed RNF187 into U87 cells. The effects of RNF187 overexpression on the growth, proliferation, cell stemness, invasion and metastasis of tumor cells in vitro were analyzed. Finally, the relationship between RNF187 and pyroptosis was studied by detecting the expressions of RNF187 and markers of pyroptosis[inflammatory body 3 (NLRP3), apoptosis associated speck like protein (ASC), GSDMD and GSDME]. Results CCK-8, cell cloning and Transwell experiments confirmed that the morphological swelling of U87 cells increased significantly after overexpression of RNF187, and the cells' ability of proliferation and invasion was significantly enhanced compared with the control group. RT-PCR and Western blot showed that the expression level of RNF187 in glioma cell line was higher than that in normal brain cells. The expression levels of NLRP3, ASC, caspase-1, GSDMD and GSDME in RNF187 the overexpression group were significantly higher than those of the control group (P＜0.05). Conclusions The higher expression of RNF187 in glioma cells can increase the proliferation and invasion of glioma cells, possibly by promoting pyroptosis.

Key words

glioma cells / ring finger protein 187 / pyroptosis / malignancy

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DONG Junqiang, PEI Meijuan, HUANG Shengxuan, LI Xuewen, TENG Chuan. Ring finger protein187 promotes the malignancy of glioma cells through pyrolytic pathway[J]. Medical Journal of the Chinese People Armed Police Forces. 2021, 32(12): 1060-1064

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