Mechanism of apoptosis of colon cancer cells induced by resveratrol through MYD88 / TLR4 / NF-KB signaling pathway

QIAO Lu; YANG Yan; TANG Daishi; DU Ting; GU Yong

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Medical Journal of the Chinese People Armed Police Forces ›› 2022, Vol. 33 ›› Issue (4) : 298-302.

ORIGINAL ARTICLES

Mechanism of apoptosis of colon cancer cells induced by resveratrol through MYD88 / TLR4 / NF-KB signaling pathway

QIAO Lu¹, YANG Yan², TANG Daishi³, DU Ting³, GU Yong³

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Abstract

Objective To investigate the mechanism of apoptosis of colon cancer cells induced by resveratrol through MyD88/TLR4/ NF-KB signaling pathway.Methods With different doses of resveratrol for 24 h, TUNEL assay was used to detect the apoptosis rate of colon cancer cells and Western blot and RT-PCR were employed to test the expression of key signal molecules in MyD88/TLR4/NF-kB signaling pathway.Results The apoptosis rates of HT-29, SW480 and LoVo significantly increased after treatment. The protein and mRNA levels of Bax and Cleaved Caspase 3 significantly increased and Bcl-2 was downregulated. The expression of VEGF protein in cell supernatant decreased significantly. MyD88, TLR4 and NF-KB were significantly down regulated (P<0.05).Conclusions Resveratrol may promote the apoptosis of colon cancer cells through MyD88/TLR4/ NF-KB signaling pathway to inhibit the development of tumor.

Key words

resveratrol / colon cancer / MyD88 / TLR4 / NF-kB

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QIAO Lu, YANG Yan, TANG Daishi, DU Ting, GU Yong. Mechanism of apoptosis of colon cancer cells induced by resveratrol through MYD88 / TLR4 / NF-KB signaling pathway[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(4): 298-302

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