Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis

SU Jinghua; JIAO Ting; HAN Ning; SU Jingke; DANG Ruijie; LI Lin; ZHANG Haisong

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Medical Journal of the Chinese People Armed Police Forces ›› 2022, Vol. 33 ›› Issue (4) : 303-306.

ORIGINAL ARTICLES

Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis

SU Jinghua¹, JIAO Ting², HAN Ning³, SU Jingke³, DANG Ruijie⁴, LI Lin⁴, ZHANG Haisong²

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Abstract

Objective To investigate the effect of cannabidiol (CBD) on podocytes MPC5 apoptosis induced by high glucose.Methods MPC5 cells were randomly divided into vehicle group, high glucose (HG) group and CBD group. CCK-8 method was used to access cell proliferation, flow cytometry was used to evaluate apoptotic rate, and western blot was used to investigate protein level of Nephrin and apoptosis-related proteins Bax and Bcl-2.Results Compared with the Vehicle group, HG significantly inhibited MPC5 proliferation and promoted MPC5 apoptosis [(25.27±2.15)% vs. (8.43±0.70)%], while CBD obviously promoted MPC5 proliferation and inhibited MPC5 apoptosis [(12.83±1.05)% vs. (25.27±2.15)%; P＜0.05] in the high glucose environment. Meanwhile, CBD administration increased Nephrin expression [(0.77±0.06) vs. (0.32±0.10)], and reduced Bax/Bcl-2 [(1.02±0.09) vs. (12.30±0.09); P＜0.05].Conclusions CBD attenuates high glucose-induced MPC5 apoptosis.

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cannabidiol / high glucose / podocyte / cell apoptosis

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SU Jinghua, JIAO Ting, HAN Ning, SU Jingke, DANG Ruijie, LI Lin, ZHANG Haisong. Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(4): 303-306

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