Application of metagenomic next-generation sequencing in diagnosis of infectious diseases of central nervous system

SHANG GUAN Lijuan; WANG Hailong; SHANG Jing; WANG Zhijun; HOU Miaomiao; XUE Lanping

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Medical Journal of the Chinese People Armed Police Forces ›› 2022, Vol. 33 ›› Issue (6) : 472-475.

ORIGINAL ARTICLES

Application of metagenomic next-generation sequencing in diagnosis of infectious diseases of central nervous system

SHANG GUAN Lijuan^1,2, WANG Hailong^1,2, SHANG Jing^1,2, WANG Zhijun^1,2, HOU Miaomiao^1,2, XUE Lanping^1,2

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Abstract

Objective To investigate the application value of metagenomic next-generation sequencing (mNGS) in the diagnosis of infectious diseases of central nervous system (CNS infectious diseases).Methods The clinical data of 85 patients with CNS infectious diseases admitted to the Neurology Department of Shanxi Bethune Hospital from January 2019 to December 2021 were retrospectively analyzed to explore the advantages of mNGS.Results Twenty-one kinds of pathogens were detected by mNGS, 57 cases were positive, 48 cases were in accordance with clinical diagnosis, 9 cases were not, and the accuracy was 56.5%(48/85); 23 cases were positive by traditional methods, 22 cases were in accordance with clinical diagnosis, 1 case was not, and the accuracy was 25.9%(22/85). The difference between the two methods was significant (P<0.05).Conclusions The detection accuracy of mNGS is higher than that of the traditional method, and mNGS can be used as a complementary method to the traditional detection one, but because of the results of mNGS are inconsistent with the clinical diagnosis in many cases, the interpretation of detection results should be carefully considered in combination with clinical practice.

Key words

metagenomic / next-generation sequencing / central nervous system infections / accuracy

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SHANG GUAN Lijuan, WANG Hailong, SHANG Jing, WANG Zhijun, HOU Miaomiao, XUE Lanping. Application of metagenomic next-generation sequencing in diagnosis of infectious diseases of central nervous system[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(6): 472-475

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