Objective To investigate the effect of hypoxia inducible factor-1 α (HIF-1α) overexpression on the malignancy of human glioma SHG44 cells and its possible mechanism.Methods Glioma cell SHG44 was cultured in vitro and transfected with HIF-1α overexpression into SHG44 cells. The effects of HIF-1α overexpression on the growth and proliferation, cell stem, invasion and metastasis of tumor cells in vitro were analyzed. Finally, the relationship between HIF-1α and pyroptosis were clarified by detecting the expression of HIF-1α, tumor-associated inflammatory factors(TNF- α, IL-10, IL-17 and IL-1 β), pyroptosis markers [inflammatory body 3 (NLRP3), apoptosis associated speck like protein (ASC), cysteinyl aspartate specific proteinase-1 (caspase-1), GSDMD, GSDME and transforming growth factor-β1(TGF-β1nd).Results MTT, cell cloning, cell scratch and Transwell experiments confirmed that the morphological swelling of SHG44 cells increased significantly after the overexpression of HIF-1α, and cell proliferation and invasion ability were significantly enhanced compared with the control group; ELISA showed that, compared with the control group, tumor inflammatory factor (TNF α,IL-10 and IL-1 β)increased significantly, while IL-17 decreased significantly; RT-PCR and Western blot showed that the expression level of HIF-1α in glioma cell line was higher than that in normal brain cells The expression levels of NLRP3, ASC, caspase-1, GSDMD and GSDME inHIF-1αoverexpression group were significantly higher than those in control group (P<0.05).Conclusions The increased expression of HIF-1α in glioma cells SHG44 can increase the proliferation and invasion of glioma cells, which may be achieved by promoting pyroptosis.
Key words
hypoxia inducible factor 1α /
glial cells /
SHG44 cells /
pyroptosis /
transfection
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