Role of ZFP580 in acute pancreatitis induced by caerulein through endoplasmic reticulum stress pathway

ZI Li; LIU Guanglin; ZHANG Wencheng; XIA Shihaii; XU Wei

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Medical Journal of the Chinese People Armed Police Forces ›› 2022, Vol. 33 ›› Issue (6) : 522-527.

ORIGINAL ARTICLES

Role of ZFP580 in acute pancreatitis induced by caerulein through endoplasmic reticulum stress pathway

ZI Li^1,2, LIU Guanglin^1,2, ZHANG Wencheng^1,2, XIA Shihaii^1,2, XU Wei^1,2

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Abstract

Objective To investigate the role of zinc finger protein 580 (ZFP580) in acute pancreatitis (AP) induced by caerulein through endoplasmic reticulum (ER) stress pathway, and provide a new therapeutic target for AP treatment.Methods MTT method was used to detect cell viability. RT-PCR and Western Blot were used to detect endoplasmic reticulum stress-related mRNA and the protein expression of ZFP580 in AP model. IRE1 inhibitor (MKC-3946) was used to observe the position of ZFP580 in endoplasmic reticulum stress related signaling pathway. After Silencing ZFP580, Hochest33342 staining and WB, RT-PCT method were used to investigate the effect of ZFP580 on the apoptosis-necrosis process.Results After using cerulein to stimulate acute pancreatitis in AR42J cells, the endoplasmic reticulum stress occurred. 8 hours later, the expression of ZFP580 started to increase (showing a time-dependent increase). After using IRE1 inhibitor (MKC-3946), the expression of ZFP580 was significantly lower than that of the group without MKC-3946 treatment (P<0.05). After silencing ZFP580, the expression of XBP-1s significantly decreased. At the same time, RT-PCR and Western blot results showed that the expression of Chop in the si-ZFP580 group was significantly higher than that in the normal AP group, while the expression of Caspase-3 was lower than that in the normal AP group. The result of Hochest staining revealed that the proportion of necrotic cells in the si-ZFP580 group was significantly higher than that in the normal AP group.Conclusions ZFP580 which may be located between IRE1 and XBP-1s may play a protective role in regulating the severity of AP by improving the apoptosis-necrosis ratio.

Key words

acute pancreatitis / cerulein / ZFP580 / ER stress

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ZI Li, LIU Guanglin, ZHANG Wencheng, XIA Shihaii, XU Wei. Role of ZFP580 in acute pancreatitis induced by caerulein through endoplasmic reticulum stress pathway[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(6): 522-527

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