Irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition

SHI Zhiyun; ZOU Rongfang; SHI Quan; CHEN Fei; ZHAI Yujie; HUANG Yang

PDF(1382 KB)

Medical Journal of the Chinese People Armed Police Forces ›› 2022, Vol. 33 ›› Issue (7) : 560-563.

ORIGINAL ARTICLES

Irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition

SHI Zhiyun, ZOU Rongfang, SHI Quan, CHEN Fei, ZHAI Yujie, HUANG Yang

Author information +

History +

Abstract

Objective To explore irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition.Methods BMSCs of rabbit were divided into 4 groups: control, HG (high glucose), irisin (HG+irisin) and CQ (chloroquine; HG+irisin+CQ). After cultured in osteogenic differentiation medium for 5 days, western blotting was used to detect autophagy-related protein levels of P62 and LC3B; qRT-PCR was used to evaluate the expression levels of Runx2, ALP, COL-I and OCN; ALP activity was also tested.Results Compared with the control group, HG group significantly increased P62 and decreased LC3B-II protein levels, down-regulated mRNA expression of Runx2, ALP and COL-I, and reduced ALP activity (P＜0.05);however, irisin obviously decreased P62 and increased LC3B-II protein levels, up-regulated mRNA expression of Runx2, ALP and COL-I, and promoted ALP activity (P＜0.05); moreover, when inhibited autophagy with CQ, the effect of irisin was blunted (P＜0.05).Conclusion Irisin promotes osteoblast differentiation of BMSCs in high glucose condition via activating autophagy.

Key words

irisin / autophagy / BMSCs / osteoblast

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SHI Zhiyun, ZOU Rongfang, SHI Quan, CHEN Fei, ZHAI Yujie, HUANG Yang. Irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(7): 560-563

References

[1] 翟羽,吴方丽,田萧羽,等. GDF11对糖尿病小鼠骨髓间充质干细胞细胞凋亡的影响及其信号机制[J]. 武警医学, 2021,32(1):19-21.
[2] 郝思维,毛小菲,胡巍,等. Ⅱ型糖尿病中BMAL1调节骨髓间充质干细胞用于骨缺损修复的研究[J]. 北京口腔医学, 2021,29(2):61-68.
[3] 李金儒,位朵,甄国朋,等. Irisin对高糖环境中BMSCs骨向分化的影响及机制研究[J]. 华南国防医学杂志, 2019,33(7):445-449.
[4] Ma Y, Qi M, An Y, et al. Autophagy controls mesenchymal stem cell properties and senescence during bone aging[J]. Aging Cell, 2018,17(1).
[5] 孙磊, 董科明, 翟羽,等. 鸢尾素通过激活自噬促进老龄小鼠BMSCs骨向分化的体外研究[J]. 口腔颌面修复学杂志, 2020,21(5):257-260.
[6] Li R, Wang X, Wu S, et al. Irisin ameliorates angiotensin II-induced cardiomyocyte apoptosis through autophagy[J]. J Cell Physiol, 2019,234(10):17578-17588.
[7] Bi J, Yang L, Wang T, et al. Irisin improves autophagy of aged hepatocytes via increasing telomerase activity in liver injury[J]. Oxid Med Cell Longev, 2020,2020:6946037.
[8] Chen X, Sun K, Zhao S, et al. Irisin promotes osteogenic differentiation of bone marrow mesenchymal stem cells by activating autophagy via the Wnt/β-catenin signal pathway[J]. Cytokine, 2020,136:155292.
[9] Ye X, Shen Y, Ni C, et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway[J]. Peptides, 2019,119:170120.
[10] Lu J, Xiang G, Liu M, et al. Irisin protects against endothelial injury and ameliorates atherosclerosis in apolipoprotein E-Null diabetic mice[J]. Atherosclerosis, 2015,243(2):438-448.
[11] Zhu B, Li Y, Mei W, et al. Alogliptin improves endothelial function by promoting autophagy in perivascular adipose tissue of obese mice through a GLP-1-dependent mechanism[J]. Vascul Pharmacol, 2019,115:55-63.
[12] 郭滨, 甄国朋, 杨海青,等. Irisin对老龄性骨质疏松小鼠骨代谢的影响及机制研究[J]. 解放军医药杂志, 2019,31(12):23-28.
[13] 朱彪, 张欣然, 赵刚,等. 以ADSCs替代BMMSCs构建组织工程骨修复种植区骨缺损的实验研究[J]. 牙体牙髓牙周病学杂志, 2015,25(11):651-658.
[14] Polyzos S A, Kountouras J, Anastasilakis A D, et al. Irisin in patients with nonalcoholic fatty liver disease[J]. Metabolism, 2014,63(2):207-217.
[15] Liu S, Du F, Li X, et al. Effects and underlying mechanisms of irisin on the proliferation and apoptosis of pancreatic β cells[J]. PLoS One, 2017,12(4):e175498.
[16] Song H, Wu F, Zhang Y, et al. Irisin promotes human umbilical vein endothelial cell proliferation through the ERK signaling pathway and partly suppresses high glucose-induced apoptosis[J]. PLoS One, 2014,9(10):e110273.
[17] Li R L, Wu S S, Wu Y, et al. Irisin alleviates pressure overload-induced cardiac hypertrophy by inducing protective autophagy via mTOR-independent activation of the AMPK-ULK1 pathway[J]. J Mol Cell Cardiol, 2018,121:242-255.
[18] 刘翠,徐晓丽,李金儒,等. 髓源性生长因子对糖尿病小鼠BMSCs骨向分化的影响及机制研究[J]. 实用口腔医学杂志, 2020,36(6):865-869.
[19] Rubinsztein D C, Mario G, Kroemer G. Autophagy and aging[J]. Cell, 2011,146(5):682-695.
[20] Wan Y, Zhuo N, Li Y, et al. Autophagy promotes osteogenic differentiation of human bone marrow mesenchymal stem cell derived from osteoporotic vertebrae[J]. Biochem Biophys Res Commun, 2017,488(1):46-52.
[21] Huang Z, Nelson E R, Smith R L, et al. The sequential expression profiles of growth factors from osteoprogenitors [correction of osteroprogenitors] to osteoblasts in vitro[J]. Tissue Eng, 2007,13(9):2311-2320.
[22] Vilchez D, Saez I, Dillin A. The role of protein clearance mechanisms in organismal ageing and age-related diseases[J]. Nat Commun, 2014,5:5659.
[23] Lee I H, Cao L, Mostoslavsky R, et al. A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy[J]. Proc Natl Acad Sci U S A, 2008,105(9):3374-3379.