Influencing factors of carrageenan to establish rat models of chronic nonbacterial prostatitis

SU Xuzhen; ZHANG Qiang; XIANG Ruijun; ZHANG Chunlei; ZHANG Bin; andCHANG Dehui

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (10) : 833-836.

ORIGINAL ARTICLES

Influencing factors of carrageenan to establish rat models of chronic nonbacterial prostatitis

SU Xuzhen^1,2,3, ZHANG Qiang^1,2,3, XIANG Ruijun^1,2, ZHANG Chunlei¹, ZHANG Bin¹, and CHANG Dehui¹

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Abstract

Objective To explore the influencing factors of carrageenan onchronic nonbacterial prostatitis (CNP) modelsin rats. Methods Adult male rats were injected with carrageenan to make models, and the effects of mouse species (SD rats and Wistar rats), injection sites (seminal vesicle andventral lobe of prostate) and injection concentration (1% and 3%) on the modeling were observed.The modeling effect was determined according to the results of HE stainingof prostate histopathological indicators. Results Compared with Wistar male rats, the results of HE staining in SD male prostate tissues were significantly higher (P<0.05). Spontaneous inflammation appeared in parts of normal Wistar male rats (P<0.05), which mightaffect the observation of later experimental results. The effect of bilateral seminal vesicle injection was better than that of left and right ventral prostatic injection(P<0.05), but it increased the mortalityof rats and acute inflammatory reaction occurred; compared with left and right ventral prostaticinjection, the successful rate of model construction was higher, and the local multi-point injection method could make up for the lackof small infiltration area.The modeling effect of 3% carrageenan saline solution was more significant than that of 1% carrageenan saline solution (P<0.05). Conclusions SD male rats are more sensitive to carrageenan than Wistar male rats. A stable and ideal CNP model will be successfully established after 21 days of local injection of 3% carrageenan saline solution into the left and right ventral lobe of the prostate of SD male rats.

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chronic prostatitis / male rats / animal model / carrageenan / model evaluation

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SU Xuzhen, ZHANG Qiang, XIANG Ruijun, ZHANG Chunlei, ZHANG Bin, andCHANG Dehui. Influencing factors of carrageenan to establish rat models of chronic nonbacterial prostatitis[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(10): 833-836

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