Effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells derived from mice in inflammatory condition

GUO Shuqin; WEN Chunfang; ZHU Biao; TIAN Xiaoyu; DANG Ruijie; WANG Lijun

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (2) : 140-143.

Effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells derived from mice in inflammatory condition

GUO Shuqin^1,2, WEN Chunfang³, ZHU Biao², TIAN Xiaoyu², DANG Ruijie², WANG Lijun⁴

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Abstract

Objective To investigate the effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells (BMSCs) in the inflammatory microenvironment.Methods BMSCs were isolated, cultured and divided into 3 groups: Vehicle, LPS and Irisin (LPS plus irisin intervention). CCK-8 was used to access cell proliferation. After cultured in osteogenic differentiation medium, ALP activity was evaluated, mRNA levels of Runx2, Osx, ALP and OCN and protein levels of ALP and OCN were also investigated. Moreover, alizarin red was used to determine mineralization level.Results Compared with the Vehicle group, LPS significantly promoted BMSCs proliferation (P＜0.05); LPS downregulated Runx2, Osx, ALP and OCN mRNA expression and reduced ALP and OCN protein levels, while irisin intervention could obviously upregulate Runx2, Osx and ALP mRNA expression, enhanced ALP and OCN protein levels, and raised mineralization level (P＜0.05). Conclusions Irisin can promote osteoblast differentiation of BMSCs in the inflammatory microenvironment.

Key words

irisin / inflammatory microenvironment / bone-marrow mesenchymal stem cells / osteoblast differentiation

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GUO Shuqin, WEN Chunfang, ZHU Biao, TIAN Xiaoyu, DANG Ruijie, WANG Lijun. Effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells derived from mice in inflammatory condition[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(2): 140-143

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