Regulation of commicroRNA-22 on chondrocyte senescence by inhibiting SIRT1 expression

YAN Shiju; DONG Wenjing; LI Zhirui; ZHAO Yanpeng; HAN Tao; WEI Junqiang; WANG Junliang; LIN Feng

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (3) : 206-209.

ORIGINAL ARTICLES

Regulation of commicroRNA-22 on chondrocyte senescence by inhibiting SIRT1 expression

YAN Shiju¹, DONG Wenjing², LI Zhirui¹, ZHAO Yanpeng³, HAN Tao¹, WEI Junqiang¹, WANG Junliang¹, LIN Feng¹

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Abstract

Objective To investigate the effects of microRNA-22 on chondrocyte senescence in osteoarthritis. Methods Primary chondrocytes were collected from OA cartilage and divided into miR-22 mimics group, miR-22 inhibitor group and Negative Control (NC) group, which were transfected with miR-22 mimics, miR-22 inhibitor and negative control respectively. MTT assay was used to detect the effects of microRNA-22 on cell proliferation of chondrocyte; senescence-associated β-galactosidase (SA-β-Gal) staining was performed to evaluate the effects of microRNA-22 on senescence of chondrocyte. Western blot was used to detect the expression level of SIRT1 and P16. Results Compared with Negative Control (NC) group, the proliferation rate of chondrocytes was significantly inhibited in miR-22 mimics group and upregulated in miR-22 inhibitor group. The ratio of SA-β-Gal-positive chondrocytes group was elevated in miR-22 mimics and downregulated in miR-22 inhibitor group. After chondrocytes were transfected with miR-22 mimics, the expression level of P16 was promoted while SIRT1 was inhibited. On the contrary, the expression level of P16 was decreased while SIRT1 was elevated after chondrocytes were transfected with miR-22 inhibitor. Conclusions For OA, microRNA-22 could inhibit chondrocyte proliferation, as well as promote chondrocyte senescence by regulating SIRT1 and P16, so microRNA-22 could be a novel target of OA treatment.

Key words

microRNA-22 / senescence / osteoarthritis / SIRT1 / P16 / chondrocyte

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YAN Shiju, DONG Wenjing, LI Zhirui, ZHAO Yanpeng, HAN Tao, WEI Junqiang, WANG Junliang, LIN Feng. Regulation of commicroRNA-22 on chondrocyte senescence by inhibiting SIRT1 expression[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(3): 206-209

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