Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism

ZHAO Xiqiang; LU Wenhua; SHANG Liqiang; DANG Ruijie; AN Ran; HE Xin

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (6) : 507-510.

Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism

ZHAO Xiqiang¹, LU Wenhua¹, SHANG Liqiang¹, DANG Ruijie², AN Ran¹, HE Xin³

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Abstract

Objective To examine the effect of myeloid-derived growth factors (MYDGF) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in high glucose environment and its molecular mechanism. Methods BMSCs were isolated and cultured in vitro, the surface markers of BMSCs were detected by flow cytometry, the osteogenic status of BMSCs was quantitatively detected by alkaline phosphatase (ALP), and the mRNA levels of OSX, RUNX2 and COL-I were detected by RT-qPCR. The protein levels of p-GSK3β and β-catenin were detected by western blot. Results ALP activity and mRNA expression of OSX, RUNX2 and COL-I in BMSCs were inhibited by high glucose (P＜0.05), and the inhibitory effect was improved after adding MYDGF (P＜0.05). The inhibitory effect of high glucose on the expression of p-GSK3β and β-catenin could be reversed by MYDGF [p-GSK3β:(0.85±0.13) vs. (0.15±0.07), β-catenin: (0.79±0.14) vs. (0.37±0.11); P＜0.05], and the promotion of MYDGF on the expression of p-GSK3β and β-catenin were inhibited after DKK1(the inhibitor of Wnt signaling pathway) treatment [p-GSK3β:(0.43±0.11) vs. (0.85±0.13), β-catenin:(0.20±0.06) vs. (0.79±0.14); P＜0.05]. Conclusions MYDGF may promote the osteogenic differentiation of BMSCs in high glucose environment by activating the Wnt/β-catenin signaling pathway.

Key words

myeloid-derived growth factor / bone marrow mesenchymal stem cells / high glucose / osteogenic differentiation / Wnt/β-catenin signaling pathway

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ZHAO Xiqiang, LU Wenhua, SHANG Liqiang, DANG Ruijie, AN Ran, HE Xin. Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(6): 507-510

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