Effects of Nrg4 on myocardial apoptosis of type 2 diabetic rats

SU Jinghua; WU Fangli; JIAO Ting; HAN Ning; SU Jingke; ZHANG Haisong

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (7) : 557-561.

Effects of Nrg4 on myocardial apoptosis of type 2 diabetic rats

SU Jinghua¹, WU Fangli², JIAO Ting³, HAN Ning⁴, SU Jingke⁴, ZHANG Haisong³

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Abstract

Objective To investigate the effects of neuregulin 4 (Nrg4) on myocardial apoptosis of type 2 diabetic rats. Methods SD rats were randomly divided into vehicle group, diabetes mellites (DM) group and Nrg4 administration group (Nrg4). The apoptosis rate of myocardial cells was detected by flow cytometry, the expression of Bax and Bcl-2 transcription were detected by qRT-PCR, and the expression of apoptosis-related proteins Bax, Bcl-2 and Cleaved caspase 3 were detected by Western blot. Results Compared with the vehicle group, DM significantly promoted cell apoptosis [(35.49±1.37)% vs. (11.98±0.85)%; P＜0.05], heightened Bax and cleaved-caspase 3 expression, and reduced Bcl-2 expression (P＜0.05). While CBD obviously inhibited cell apoptosis [(19.28±0.96)% vs. (35.49±1.37)%; P＜0.05], reduced Bax and cleaved-caspase 3 expression, and increased Bcl-2 expression (P＜0.05). Conclusions Nrg4 attenuates myocardial apoptosis of type 2 diabetic rats.

Key words

Neuregulin 4 (Nrg4) / type 2 diabetes / cardiomyocyte / cell apoptosis

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SU Jinghua, WU Fangli, JIAO Ting, HAN Ning, SU Jingke, ZHANG Haisong. Effects of Nrg4 on myocardial apoptosis of type 2 diabetic rats[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(7): 557-561

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