Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress

LEI Jing; ZHANG Xinyu; CHEN Lijun; WANG Daning; MENG Bin; WEN Xiaochang; WANG Jiangping

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Medical Journal of the Chinese People Armed Police Forces ›› 2023, Vol. 34 ›› Issue (9) : 794-798.

ORIGINAL ARTICLES

Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress

LEI Jing¹, ZHANG Xinyu¹, CHEN Lijun¹, WANG Daning², MENG Bin³, WEN Xiaochang¹, WANG Jiangping¹

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Abstract

Objective To explore the effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress.Methods Twenty-four male SD rats were randomly divided into a control group, a model group and a resistant dextrin group. A depression-like rat model was established using chronic unpredictable mild stress (CUMS). After 3 weeks of stress modelling, sucrose preference test and open- field test were used to evaluate the depression-like behavior of rats. Stress sensitive rats were screened in the model group, and the same number of rats in the other two groups were randomly selected to detect the composition and structural changes of gut microbiota by 16S rRNA sequencing.Results Compared with the control group, relative abundance of Firmlcutes [(0.92251±0.02838) vs. (0.82572±0.00761)], Akkermansia[(0.01327±0.00076) vs. (0.00000±0.00000)]and Lactococcus [(0.00029±0.00010) vs. (0.00000±0.00000)] in the model group was significantly reduced (P<0.05); relative abundance level of Bacteroidetes (0.04671±0.00263) vs. (0.12341±0.03576), Faecalibacterium[(0.00011±0.00004)vs.(0.00057±0.00005)]and Ruminococcaceae_UCG_014 [(0.01003±0.00384)vs.(0.10976±0.04364)] was significantly increased (P<0.05). Compared with the model group, resistant dextrin could significantly callback the microflora with changed abundance (P<0.05).Conclusions Resistant dextrin can regulate the gut microbiota of CUMS rats and restore intestinal dynamic balance by improving the relative abundance of probiotics and reducing the relative abundance of pathogenic bacteria, which provides experimental basis for the treatment of depression.

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chronic unpredictable mild stress / gut microbiota / resistant dextrin / depression

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LEI Jing, ZHANG Xinyu, CHEN Lijun, WANG Daning, MENG Bin, WEN Xiaochang, WANG Jiangping. Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(9): 794-798

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