Effects of myeloid-derived growth factor on insulin resistance and inflammatory response of liver from type 2 diabetic mice

SU Fang; FU Zhiguang; FANG Wencan; WANG Ying; HE Mingjuan; ZHANG Yongbin

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Medical Journal of the Chinese People Armed Police Forces ›› 2024, Vol. 35 ›› Issue (3) : 201-204.

Effects of myeloid-derived growth factor on insulin resistance and inflammatory response of liver from type 2 diabetic mice

SU Fang¹, FU Zhiguang¹, FANG Wencan², WANG Ying², HE Mingjuan³, ZHANG Yongbin⁴

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Abstract

Objective To investigate the effects of myeloid-derived growth factor (MYDGF) on insulin resistance and inflammatory response of liver from type 2 diabetic mice. Methods C57 mice were randomly divided into Vehicle group, DM group and MYDGF group. After administration for 12 weeks, fasting blood glucose (FBG), insulin and HOMA-IR were determined. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and Catalase in the liver were measured. Morphological changes of liver were assessed by HE staining. Phosphorylation levels of IKKβ and IkBα were detected by Western blot. Results Compared with DM group, the levels of FBG[(6.91±0.71) mmol/L vs. (19.46±1.12) mmol/L], and insulin in MYDGF group[(13.29±1.42) Mu/L vs. (21.88±1.58) Mu/L] and HOMA-IR[(4.08±0.65) vs. (18.90±1.37)] obviously decreased (P<0.05). The concentrations of TNF-α[(32.17±3.18)ng/L vs. (66.39±4.51) ng/L] and IL-6 [(13.65±0.89) ng/L vs. (21.55±3.27) ng/L] also significantly decreased (P<0.05). Liver SOD [(297.54±20.43) U/mg vs. (198.32±19.29) U/mg], GSH-Px[(0.65±0.05) U/mg vs. (0.19±0.04) U/mg] and Catalase [(220.34±19.82) U/mg vs. (134.69±18.81) U/mg] levels increased (P<0.05); in addition, inflammatory cell infiltration and hepatocyte steatosis attenuated; lastly, phosphorylation levels of IKKβ [p-IKKβ:IKKβ, (0.97±0.10) vs. (1.39±0.11)] and IkBα [p-IκBα:IκBα, (0.93±0.07) vs. (1.44±0.06)] significantly increased (P＜0.05) in MYDGF group. Conclusions MYDGF improves insulin resistance, which may be attributed to attenuated inflammatory response from diabetic liver via activating the IKKβ/IkBα signaling pathway.

Key words

myeloid-derived growth factor / IKKβ/IkBα signaling pathway / type 2 diabetes / insulin resistance

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SU Fang, FU Zhiguang, FANG Wencan, WANG Ying, HE Mingjuan, ZHANG Yongbin. Effects of myeloid-derived growth factor on insulin resistance and inflammatory response of liver from type 2 diabetic mice[J]. Medical Journal of the Chinese People Armed Police Forces. 2024, 35(3): 201-204

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