Effects of neuregulin 4 on apoptosis of aortic endothelial cells in type 2 diabetic mice

LIU Xingyu; WANG Ying; JIAO Ting; FANG Wencan; YU Hanying; ZHANG Haisong

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Medical Journal of the Chinese People Armed Police Forces ›› 2024, Vol. 35 ›› Issue (7) : 593-596.

ORIGINAL ARTICLES

Effects of neuregulin 4 on apoptosis of aortic endothelial cells in type 2 diabetic mice

LIU Xingyu¹, WANG Ying², JIAO Ting³, FANG Wencan², YU Hanying², ZHANG Haisong³

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Abstract

Objective To investigate the effects of neuregulin 4 (Nrg4) on the apoptosis of endothelial cells in type 2 diabetic mice. Methods C57 mice were randomly divided into Vehicle group, diabetes mellites (DM) group and Nrg4 administration group (NRG4). Nrg4 group was intraperitoneally (i.p.) injected with Nrg4 recombinant protein (100 μg/kg, 3 times/week) and the same volume of saline was i.p. injected in the other two groups for 8 weeks. After the experiment, TUNEL staining was used to evaluate apoptotic rate of endothelial cells, and Western blot was used to investigate the protein levels of apoptosis-associated proteins. Results Compared with Vehicle group, DM significantly promoted cell apoptosis [(33.0±5.4)% vs. (20.5±3.0)%;P<0.05], increased Bax and cleaved-caspase 3 expression, and decreased Bcl-2 expression (P<0.05), while Nrg4 administration obviously decreased endothelial apoptosis [(19.2±3.8) % vs. (33.0±5.4) %; P<0.05], decreased Bax and cleaved-caspase 3 expression, and increased Bcl-2 expression (P<0.05). Conclusions Nrg4 administration can alleviate the apoptosis of aortic endothelial cells in type 2 diabetic mice.

Key words

neuregulin 4 / diabetes mellitus / endothelial cell / cell apoptosis

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LIU Xingyu, WANG Ying, JIAO Ting, FANG Wencan, YU Hanying, ZHANG Haisong. Effects of neuregulin 4 on apoptosis of aortic endothelial cells in type 2 diabetic mice[J]. Medical Journal of the Chinese People Armed Police Forces. 2024, 35(7): 593-596

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