Objective To explore the relationship between long-chain non-coding ribonucleic acids (LncRNAs) and radiotherapy efficacy of lung squamous cell carcinoma. Methods LncRNAs were used as the exposure factor, and the radiotherapy effect of lung squamous cell carcinoma was used as the outcome event. Two-sample Mendelian randomization analysis method was adopted to analyze the Mendelian randomization association between LncRNAs and the radiotherapy effect of lung squamous cell carcinoma from the GWAS datasets obtained from the genome wide association studies (GWAS) database. The inverse variance weighting method (IVW) was used as the main analysis method, and MR-Egger regression, weighted median estimator (WME), simple model (SM) and weighted model (WM) were used as the secondary analysis methods. Heterogeneity test and sensitivity analysis were conducted to ensure the robustness and reliability of the results. Results The IVW analysis showed that long chain non-coding ribonucleic acid large intergenic noncoding 00261 (LncRNA linc00261) (OR=0.442, 95% CI: 0.245-0.799) was a protective factor for the ineffective radiotherapy of lung squamous cell carcinoma (P<0.05), LncRNA HOX anti-sense RNA (HOTAIR) (OR=1.768, 95%CI: 1.050-2.978), and LncRNA H19 (OR=1.749, 95%CI: 1.324-2.310) were risk factors for the ineffective radiotherapy of lung squamous cell carcinoma (P<0.05), and the β values analyzed by MR Egger, WME, SM, and WM were consistent with that of IVW. The Cochran’s Q heterogeneity test showed that there was no heterogeneity in the SNPs of LncRNA HOTAIR, LncRNA linc00261, and LncRNA H19 (P>0.05). The intercept analysis of MR Egger regression showed that no horizontal pleiotropy was detected in the SNPs of the above three LncRNAs(P>0.05). The Mendelian randomization pleiotropy residual sum and outlier (MR-PRESSO) method analysis showed that there were no outliers in SNPs highly correlated with the three LncRNAs mentioned above(P>0.05). The sensitivity analysis results of the "leave one method" showed that after removing the SNPs of the three LncRNAs strongly related to the radiotherapy effect of lung squamous cell carcinoma one by one, there was no significant change in the two-sample Mendelian randomization analysis results. Conclusions LncRNA linc00261 is a protective factor for the ineffective radiotherapy of lung squamous cell carcinoma, reducing the risk of ineffective radiotherapy, while LncRNA HOTAIR and LncRNA H19 are risk factors for the ineffective radiotherapy of lung squamous cell carcinoma, increasing the risk of ineffective radiotherapy.
Key words
long chain non-coding ribonucleic acid /
lung squamous cell carcinoma /
radiotherapy /
Mendelian randomization analysis
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