Construction of a composite nanofilm for oral use based on dopamine-grafted methacrylated gelatin

LIU Xin; REN Yipeng

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Medical Journal of the Chinese People Armed Police Forces ›› 2025, Vol. 36 ›› Issue (12) : 1025-1030.

Construction of a composite nanofilm for oral use based on dopamine-grafted methacrylated gelatin

LIU Xin¹, REN Yipeng²

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Abstract

Objective To construct a composite nano-film for oral use based on biocompatible and bioactive materials with abundant active sites grafted with adhesion groups and functional factors, and to provide a new approach for the treatment of oral mucosal wounds through studying its mechanical and biological properties. Methods A composite nanofilm was prepared using methacrylated gelatin (GelMA), dopamine (DA), antimicrobial peptides (AMP), and calcium ions (Ca²⁺) as the base materials by solution spin coating. The mechanical properties were evaluated by tensile stress and swelling stress tests. The biocompatibility was detected by cell viability and CCK-8 proliferation assays. The in vitro antibacterial ability of the composite films was determined by plate inhibition tests and SEM. The in vitro hemostatic performance of the composite nanofilms was analyzed by the platelet method. The wet adhesion ability was investigated by in vitro adhesion tests. Results GelMA, DA, AMP and Ca²⁺ were selected as the base materials, and an independent nanofilm was fabricated by solution spin-coating method. The pressure tolerance reached (6.75±0.25) kPa, which was higher than human blood pressure. The maximum tensile stress was 4 MPa, close to that of human soft tissues. Cell experiments confirmed good biocompatibility. The bactericidal efficiency exceeded 99% within 2 h. It exhibited excellent wet adhesion performance in simulated oral moist environment. Conclusions A composite nanofilm with wet adhesion, antibacterial and hemostatic properties for oral use has been successfully constructed for oral use, providing an innovative solution for the closure and repair of oral mucosal wounds.

Key words

oral nanofilm / oral mucosal wound / methacrylated gelatin / dopamine / wet adhesion

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LIU Xin, REN Yipeng. Construction of a composite nanofilm for oral use based on dopamine-grafted methacrylated gelatin[J]. Medical Journal of the Chinese People Armed Police Forces. 2025, 36(12): 1025-1030

References

[1] Toma A I, Fuller J M, Willett N J, et al. Oral wound healing models and emerging regenerative therapies[J]. Transl Res, 2021, 236:17-34.
[2] Nikoloudaki G, Creber K, Hamilton D W. Wound healing and fibrosis: a contrasting role for periostin in skin and the oral mucosa[J]. Am J Physiol Cell Physiol, 2020,318(6):C1065-C1077.
[3] 陈谦明.口腔黏膜病学[M].北京:人民卫生出版社,2016:12-197.
[4] Kong X, Fu J, Shao K, et al. Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa[J]. Acta Biomater,2019,100:255-269.
[5] Chen Y, Gao B, Cai W, et al. Oral mucosa: anti-inflammatory function, mechanisms, and applications[J]. J Mater Chem B, 2025,13(13):4059-4072.
[6] Kim Y J, Carvalho F C, Souza J A,et al. Topical application of the lectin artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production[J]. Wound Repair Regen, 2013, 21(3):456-463.
[7] Lau C B, Smith G P. Recurrent aphthous stomatitis: a comprehensive review and recommendations on therapeutic options[J]. Dermatol Ther,2022,35(6):e15500.
[8] Liu H, Tan L, Fu G,et al. Efficacy of topical intervention for recurrent aphthous stomatitis: a network meta-analysis[J]. Medicina (Kaunas),2022,58(6):771.
[9] Bayda S, Adeel M, Tuccinardi T, et al. The history of nanoscience and nanotechnology: from chemical-physical applications to nanomedicine[J]. Molecules, 2019,25(1):112.
[10] Blanco-F B, Castaño O, Mateos M Á, et al. Nanotechnology approaches in chronic wound healing[J]. Adv Wound Care (New Rochelle),2021,234-256.
[11] 杨汇尚.用于难愈伤口修复的功能化纳米膜的制备及其性能研究[D].广州:华南理工大学, 2020.
[12] Tripathy D B, Gupta A. Nanomembranes-affiliated water remediation: chronology, properties, classification, challenges and future prospects[J]. Membranes (Basel),2023,13(8):713.
[13] Krysztofik A, Warzajtis M, Pochylski M. Multi-responsive poly-catecholamine nanomembranes[J]. Nanoscale,2024,16(34):16227-16237.
[14] Deng Daokun,Li Xuan,Zhang JiuJiu,et al. Biotin-avidin system-based delivery enhances the therapeutic performance of MSC-derived exosomes.[J].ACS Nano,2023,17(9):8530-8550.
[15] Fu Y, Yang L, Zhang J, et al. Polydopamine antibacterial materials[J]. Mater Horiz, 2021, 8(6):1618-1633.
[16] Yazdi M K, Zare M, Khodadadi A, et al. Polydopamine biomaterials for skin regeneration[J]. ACS Biomater Sci Eng,2022,8(6):2196-2219.
[17] Zheng P, Ding B, Li G. Polydopamine-incorporated nanoformulations for biomedical applications[J]. Macromol Biosci,2020,20(12):e2000228.
[18] El Yakhlifi S, Ball V. Polydopamine as a stable and functional nanomaterial[J]. Colloids Surf B Biointerfaces,2020,186:110719.
[19] Xu Z, Wang T, Liu J. Recent development of polydopamine anti-bacterial nanomaterials[J]. Int J Mol Sci,2022,23(13):7278.
[20] Moreira J, Vale A C, Alves NM. Spin-coated freestanding films for biomedical applications[J]. J Mater Chem B,2021,9(18):3778-3799.
[21] Powojska A, Mystkowski A, Gundabattini E, et al. Spin-coating fabrication method of PDMS/NdFeB composites using chitosan/PCL coating[J]. Materials (Basel),2024,17(9):1973.
[22] Yin Z, Tian B, Zhu Q, Duan C. Characterization and application of PVDF and its copolymer films prepared by spin-coating and langmuir-blodgett method[J]. Polymers (Basel), 2019, 11(12):2033.
[23] Hernandez G, Messina A, Kattan E. Invasive arterial pressure monitoring: much more than mean arterial pressure[J]. Intensive Care Med,2022,48(10):1495-1497.
[24] Han L, Lu X, Liu K, et al. Mussel-inspired adhesive and tough hydrogel based on nanoclay confined dopamine polymerization[J]. ACS Nano,2017,11(3):2561-2574.
[25] Li Y, Yu P, Wen J, et al.Nanozyme-based stretchable hydrogel of low hysteresis with antibacterial and antioxidant dual functions for closely fitting and wound healing in movable parts[J].Adv Funct Mater,2022, 32(13): 2110720.
[26] He Y, Liu K, Guo S, et al. Multifunctional hydrogel with reactive oxygen species scavenging and photothermal antibacterial activity accelerates infected diabetic wound healing[J]. Acta Biomater,2023,155:199-217.
[27] Chen Z, Luo L, Luo K, et al. Bioactive materials in periodontal regeneration[J]. Chin J Biotechnol, 2024, 40(2): 378-390.