Effects and mechanism of platelet-rich plasma on the proliferation and differentiation of skin cells

GUO Xiaojin; MA Chenkai; SHU Xin; PAN Jichun

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Medical Journal of the Chinese People Armed Police Forces ›› 2025, Vol. 36 ›› Issue (5) : 384-387.

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Effects and mechanism of platelet-rich plasma on the proliferation and differentiation of skin cells

GUO Xiaojin¹, MA Chenkai¹, SHU Xin², PAN Jichun¹

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Abstract

Objective To explore the effects of platelet-rich plasma (PRP) on the proliferation and differentiation of skin cells and its mechanisms of action. Methods Human dermal fibroblasts（HDFs）and human epidermal keratinocytes（HEKs) were cultured in vitro and subjected to varying concentrations of PRP at different concentrations. Cell counting kit-8（CCK-8), immunofluorescence staining and enzyme-linked immunosorbent assay（ELISA) techniques were utilized to evaluate cell proliferation, differentiation, and the expression of associated growth factors, and the effects of different concentrations of PRP on HDFs and HEKs were systematically evaluated. Results PRP significantly enhanced the proliferation rates of HDFs and HEKs,facilitated cell differentiation,and upregulated the expression of growth factors such as platelet derived growth factor（PDGF),epidermal growth factor（EGF),and fibroblast growth factor（FGF) , and the differences were statistically significant（P<0.05). Conclusions PRP can increase the expression level of growth factors in promoting the proliferation and differentiation of skin cells.

Key words

platelet-rich plasma / skin cells / proliferation / differentiation / growth factors

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GUO Xiaojin, MA Chenkai, SHU Xin, PAN Jichun. Effects and mechanism of platelet-rich plasma on the proliferation and differentiation of skin cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2025, 36(5): 384-387

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