Objective To analyze the main active components in the serum of Banxia Xiexin decoction were analyzed,and its antibacterial effect in vitro. Methods After decocting and concentrating Banxia Xiexin decoction,the drug-containing serum of Banxia Xiexin decoction was prepared by intragastric administration to rats. The main drug active components in the serum were determined by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). According to the results of HPLC-MS-MS results,the inhibitory effects of main active components and drug-containing serum of Banxia Xiexin decoction on Helicobacter pylori and other bacteria were detected by microbroth dilution method in vitro. Results The test results showed that the contents of the main active components in the drug-containing serum, from high to low, were quercetin, isorhamnetin, baicalin, succinic acid, and codonopsis glycoside. The results of the in vitro antibacterial experiments showed that minimum inhibitory concentration (MIC) of codonopsis pilosula for inhibiting bacteria （staphylococcus aureus, staphylococcus albicans, escherichia coli, salmonella, proteus, helicobacter pylori） was 100μg/ml.,the MIC of isorhamulin was 400 μg/ml for helicobacter pylori,and the remaining bacteria were 200 μg/ml. The MIC of baicalin in helicobacter pylori was 400 μg/ml,and others were 200 μg/ml. The MIC of quercetin was 200 μg/ml. The MIC of drug containing serum was in a volume ratio of 1：4 （staphylococcus aureus,escherichia coli and proteus） and 1：1 （salmonella and helicobacter pylori）. Succinic acid did not inhibit the growth of these bacteria. Conclusion The main active components of the drug-containing serum in the modified formula of Banxia Xiexin decoction, namely codonopsis glycoside,baicalin,quercetin and isorhamnine,have the effect of inhibiting helicobacter pylori and other bacteria, clarifying the main reason the main reasons for the antibacterial and anti-inflammatory effects of the modified formula of Banxia Xiexin decoction.
Key words
Banxia Xiexin decoction /
high performance liquid chromatography tandem mass spectrometry /
minimum inhibitory concentration /
medicated serum
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References
[1] Bai Y,Chen Y C,Chen Y,et al.Efficacy of Banxia Xiexin decoction in a rat model of chronic atrophic gastritis[J]. J Tradit Chin Med,2019,39(6):867-874.
[2] Li B,Rui J,Ding X,et al.Exploring the multicomponent synergy mechanism of Banxia Xiexin Decoction on irritable bowel syndrome by a systems pharmacology strategy[J].J Ethnopharmacol,2019,233 :158-168.
[3] Yu Y,Zhang G,Han T,et al.Analysis of the pharmacological mechanism of Banxia Xiexin Decoction in treating depression and ulcerative colitis based on a biological network module[J].BMC Complement Med,2020,20(1):199.
[4] Kim H R,Lee G S,Kim M S,et al.Effects of Banxia Xiexin Decoction on cisplatin-induced apoptosis of human A549 lung cancer cells[J].Chin J Integr Med,2018,24(6):436-441.
[5] Li K,Xu G,Liu C,et al.Effect of a modified Banxia Xiexin Decoction plus chemotherapy on stage III colon cancer[J].J Tradit Chin Med,2019,39(2):251-257.
[6] 李永东. 高效液相色谱法测定复方鱼腥草胶囊中黄芩苷和黄芩素[J].锦州医科大学学报,2018,39(3):7-9.
[7] 蔡迪鸣. HPLC法同时测定党参中烟酸及党参炔苷含量的方法探究[J].医学前沿,2018,8(18):398.
[8] 张学英,章发盛,黄继红.高效液相色谱法同时测定青钱柳叶中异槲皮素等5种黄酮单体的含量研究[J].中国标准化,2019,4:153-157.
[9] 顾祎雯,宋秀道,周梁,等.半夏泻心汤剂对3-脱氧葡萄糖醛酮诱导的糖尿病前期大鼠的影响[J].中国临床药理学杂志,2022,38(19):2320-2323.
[10] 汪健,朱阳,许恒,等.HPLC同时测定半夏泻心汤中8种成分[J].安徽科技学院学报,2018,32(5): 53-57.
[11] 郭迎超,梁爽,周波.中药含药血清制备的思考与探讨[J].云南中医中药杂志,2021,42(9):51-55.
[12] 周祯祥,杨伟峰,陈泽斌,等.细辛含药血清制备方法的研究[J].湖北中医杂志,2007,29(12):5-6.
[13] Wang S,Zhang J,Zhang Z,et al.Identification of chemical constituents in the extract and rat serum from ziziphus jujuba mill by HPLC-PDA-ESI-MSn[J].Iran J Pharm Res,2014,13(3):1055-1063.
[14] Wang Y,Zhang Y,Xiao J,et al.Simultaneous determination of baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re of Banxia Xiexin decoction in rat plasma by LC-MS/MS and its application to a pharmacokinetic study[J].Biomed Chromatogr,2018,32(2):124.
[15] Moore R D,Lietman P S,Smith C R.Clinical response to aminoglycoside therapy: importance of the ratio of peak concentration to minimal inhibitory concentration[J].J Infect Dis,1987,155(1):93-99.
[16] 李晏乐. 清热解毒燥湿方足部抑菌作用的体外抑菌实验研究[D].北京:中国中医科学院,2019.
[17] 陆婷婷. 半夏泻心汤活性成分对实验性胃溃疡粘膜下修复机制研究[D].广州:暨南大学,2006.
[18] Sun Y,Qu H H,Sai J Y,et al.Determination of baicalin and ginsenoside Re in Banxia Xiexin decoction using pharmacokinetics and icELISA analysis in mice.Effects of interaction between prescription herbs on the pharmacokinetics of compounds[J].Anal Methods,2015,7(7):3048-3053.
[19] 饶怡菲,江志辉,何明珍,等.基于超高效液相色谱-串联质谱法研究宽缨酮在大鼠体内的生物利用度及代谢产物[J].中药新药与临床药理,2020,31(4):455-463.
[20] Liu B,Song Z,Yu J,et al.The atherosclerosis-ameliorating effects and molecular mechanisms of Bu Yang Huan Wu decoction[J].Biomed Pharmacother,2020,123:109664.
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