目的 探讨复方中药汤剂联合电针治疗大鼠非酒精性脂肪肝(NAFLD)的疗效及安全性。方法 采用高脂饮食(HFD)联合腹腔注射四氯化碳(CCl4)玉米油混合液建立NAFLD大鼠模型。造模8周后将造模成功大鼠50只随机分为5组(n=10):高脂饮食组、正常饮食组、电针组、复方中药汤剂组及联合治疗组,每组10只。HFD组持续高脂饲料喂养;ND组改为正常饲料喂养;EA组选取双侧“足三里”(ST 36)、“丰隆”(ST 40)、“三阴交”(SP 6)、“太冲”(LR 3)及尾部非穴位进行电针治疗,1次/d,每周5 d;TCM组予复方中药汤剂1 ml/100 g灌胃,2次/d;EA-TCM组同时给予电针及中药汤剂治疗,连续干预4周。采用HE染色观察肝组织病理形态,并分别检测肝功能指标、血脂指标、氧化应激指标、糖代谢指标及肾功能指标。结果 与HFD组相比,各治疗组肝组织脂肪变性及气球样变均减轻,其中联合治疗组效果最显著。血脂方面,联合治疗组总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL)水平显著降低,HDL-C显著升高(P<0.05);EA组与TCM组效果相当。肝功能方面,联合治疗组丙氨酸氨基转移酶( ALT)及天冬氨酸氨基转移酶(AST)活性显著降低(P<0.05)。氧化应激方面,联合治疗组T-SOD活性显著升高、丙二醛含量显著降低(P<0.05)。糖代谢方面,EA组、TCM组及联合治疗组血糖水平均显著降低(P<0.05);各组糖化血清蛋白无显著差异。安全性方面,各组对肾功能指标血尿素氮、血肌酐均无显著差异。结论 复方中药汤剂及电针治疗对NAFLD大鼠均有一定的疗效,联合治疗效果更为显著。
Abstract
Objective To investigate the therapeutic efficacy of a compound traditional Chinese medicine decoction combined with electroacupuncture in a rat model of non-alcoholic fatty liver disease(NAFLD). Methods An NAFLD rat model was established through high-fat diet(HFD) feeding plus intraperitoneal injection of carbon tetrachloride(CCl4) corn oil mixture. After 8 weeks of modeling, successfully modeled rats were randomized into five groups(n=10): HFD group, normal diet(ND) group, electroacupuncture(EA) group, compound traditional Chinese medicine decoction(TCM) group, and combined therapy(EA-TCM) group. The HFD group received continuous HFD feeding; the ND group switched to normal diet; the EA group underwent electroacupuncture at bilateral “Zusanli”(ST 36), “Fenglong”(ST 40), “Sanyinjiao”(SP 6), “Taichong”(LR 3) and a non-acupoint on the tail, once daily for 5 days per week; the TCM group received intragastric administration of compound traditional Chinese medicine decoction at 1 ml/100 g twice daily; the EA-TCM group received both electroacupuncture and decoction for 4 consecutive weeks. Liver histopathology was observed through HE staining. Serum indicators including liver function, blood lipids, oxidative stress, glucose metabolism and renal function were measured. Results Compared with the HFD group, all treatment groups showed reduced hepatic steatosis and ballooning degeneration, with the combined therapy group demonstrating the most pronounced improvement. For blood lipids, the EA-TCM group exhibited significantly decreased TC, TG and LDL-C levels and elevated HDL-C(P<0.05), while the EA and TCM groups showed comparable effects. Regarding liver function, the EA-TCM group displayed markedly reduced ALT and AST activities(P<0.05). In terms of oxidative stress, the EA-TCM group had significantly increased T-SOD activity and decreased MDA content(P<0.05). For glucose metabolism, the EA, TCM and EA-TCM groups all showed significantly lowered GLU levels(P<0.05), whereas no significant differences emerged in GSP across groups. Safety assessment revealed no significant differences in renal function indicators BUN and CREA among all groups. Conclusion Both the compound traditional Chinese medicine decoction and electroacupuncture confer measurable therapeutic benefit against NAFLD in rats, with their combined application yielding superior efficacy.
关键词
复方中药汤剂 /
电针 /
联合疗法 /
非酒精性脂肪肝 /
疗效
Key words
compound traditional Chinese medicine decoction /
electroacupuncture /
combination therapy /
non-alcoholic fatty liver disease /
efficacy
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基金
上海市医疗卫生科研专项课题(CNKW2022Y)
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