目的 为探索抑郁症的发病机制及其基因诊断和治疗提供科学的依据。方法 分析药物效应动力学Mμ阿片受体基因OPRM1(Mμ-Opioid receptor,OPRM1)的遗传多态性数据。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和聚合酶链反应-序列特异性引物(PCR-SSP)分析技术检测369例抑郁症患者与738例健康部队官兵OPRM1基因第一外显子区rs1799971C/T与第三内含子区rs648893G/A两个多态性位点的基因型和等位基因频率。运用SPSS13.0和Haploview等软件进行统计学分析。结果 OPRM1基因位点的基因型及等位基因频率分布在抑郁症组与正常对照组间存在统计学意义(P<0.05)。其中,rs1799971A/G的等位基因频率只在男性34～49岁抑郁症组与对照组间存在统计学差异(OR=0.564,95%CI:0.196～0.837,P=0.001),而rs648893C/T位点的等位基因频率在不同年龄的病例组与对照组间不存在统计学意义差异(OR=0.853,95%CI:0.649～1.121,P=0.139)。结论 OPRM1基因第一外显子区rs1799971位点多态性可能是中国北方汉族抑郁症患者的风险因素,可能与抑郁症的发病存在关联。

Objective To investigate the pharmacodynamics of Mμ-Opioid receptor gene(OPRM1)as a candidate gene to obtain the data on genetic polymorphisms for the study of depression pathogenesis, genetic diagnosis and treatment.Methods Genomic DNA was isolated from the venous blood leukocytes of 393 unrelated patients with depressive disorder and 738 unrelated healthy servicemen.All the polymorphisms were genotyped by PCR-restriction fragment length polymorphisms (PCR-RFLP)and polymerase chain reaction-sequence specific primer(PCR-SSP).Genotypes and allele frequencies were analyzed with SPSS13.0 software and Haploview software.Results There was significant difference in both allele and genotype frequencies of OPRM1 genes between the depressive disorder group and control group(P<0.05).The allele T of rs1799971C/T in the male depressive disorder group aged 34 to 49 was significantly higher than that in the control group(OR=0.564,95%CI:0.196-0.837,P=0.001) The allele A of rs648893G/A was not significantly different between the depressive disorder group and the control group(OR=0.853,95%CI:0.649-1.121,P=0.139).Conclusions OPRM1genes are associated with depressive disorder with exon1 T allele of rs1799971.The OPRM1 gene is susceptible to depressive disorder in northern Han Chinese populations.