目的 探讨胎盘生长因子(placenta growth factor,PlGF)在慢性哮喘大鼠肺组织中的表达。方法 45只雄性SD大鼠随机分为3组(每组15只):(1)正常大鼠组(A组):未行特殊干预处理;(2)卵清蛋白(ovalbumin,OVA)致敏及生理盐水吸入大鼠组(B组):应用OVA致敏生理盐水激发;(3)慢性哮喘大鼠组(C组):应用OVA致敏和反复激发制备大鼠慢性哮喘模型。各组大鼠于末次激发后24 h处死。ELISA法测定肺泡灌洗液(BALF)中PlGF水平;大鼠肺组织标本行HE染色、PAS染色及Masson三色染色,并行病理图像形态学测定和分析;免疫组化检测大鼠气道上皮细胞PlGF的表达。结果 慢性哮喘大鼠组(C组)气道上皮细胞PlGF的表达(PI为2.28±0.18)与正常大鼠组(A组)及OVA致敏生理盐水吸入大鼠组(B组),(PI分别为0.89±0.08、0.94±0.12)相比明显增多(A组与C组比较P<0.01,B组与C组比较P<0.01)。慢性哮喘大鼠组(C组)BALF中PlGF水平(18.87±4.53)ng/ml与正常大鼠组(A组)及OVA致敏生理盐水吸入大鼠组(B组),分别为(12.35±1.94)ng/ml、(13.14±2.52)ng/ml相比明显增高(P<0.05)。结论 胎盘生长因子(PlGF)在慢性哮喘大鼠肺组织中表达增多。
Abstract
Objective To investigate the expression of placenta growth factor (PlGF) in lung tissue of chronic asthma rats.Methods Forty-five male Wistar rats were randomly divided into three groups. Rats in group A were given no special treatment.Rats in group B were sensitized with OVA and challenged with saline.Rats in group C were sensitized and challenged with OVA to establish a chronic asthma model. The expression of PlGF in BALF was detected using ELISA.Lung sections were stained with hematoxylin and eosin (HE), periodic acid Schiff (PAS), and Masson trichrome, and the images were analyzed morphometrically. The expression of PlGF in the airways was detected withimmunohistochemistry.Results The expression of PlGF in the airways of rats in group C was up-regulated more significantly than in group A and group B(*P<0.01, **P<0.01),and more significantly in BALF of chronic asthma rats (group C) than in group A and group B(P<0.05).Conclusions The expression of PlGF is upregulated in the lung tissue of chronic asthma rats.
关键词
胎盘生长因子 /
支气管哮喘 /
肺泡灌洗液
Key words
placenta growth factor /
asthma /
bronchus alveolus lavage fluid
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基金
福建中医药大学校管科研课题(XB2017078)
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