目的 探究光动力疗法联合环丙沙星治疗海水浸泡伤的效果。方法 将50只BALB/c小鼠随机分为清洁伤口对照组(Control组)、海水浸泡伤模型组(SII组)、海水浸泡伤光动力治疗组(PDT组)、海水浸泡伤环丙沙星治疗组(CPFX组)、海水浸泡光动力联合环丙沙星治疗组(PDT+CPFX组),每组10只。PDT组小鼠进行1次光动力治疗20 min(功率密度15 mW/cm2),CPFX组小鼠每24 h伤口表面涂抹环丙沙星软膏,连续治疗7 d,PDT+CPFX组小鼠进行1次光动力治疗20 min后再每24 h伤口表面涂抹环丙沙星软膏,环丙沙星软膏连续治疗7 d。实验第0、3、7、10和14天拍照记录各组小鼠伤口大体变化。实验第3、7和10天通过苏木精伊红(HE)染色观察各组小鼠创面病理变化,以及光动力治疗是否会引起小鼠心脏、肝脏、脾脏、肺脏和肾脏组织发生病理变化。实验第3、7和10天通过免疫组织化学(IHC)检测各组小鼠创面肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)含量。结果 与Control组相比,SII组小鼠创面愈合延迟,炎性浸润明显,炎症因子TNF-α和IL-6含量升高(P<0.05);与SII组相比,PDT+CPFX组小鼠创面愈合效果最佳,且在第10天就表现出明显的表皮重建和较少的炎性细胞浸润(P<0.05),光动力治疗不会对小鼠心脏、肝脏、脾脏、肺脏、肾脏和脑产生明显影响。结论 光动力治疗联合环丙沙星具有协同促进海水浸泡创面愈合的效果。
Abstract
Objective To investigate the effect of photodynamic therapy combined with ciprofloxacin in the treatment of seawater immersion injury. Methods A total of 50 BALB/c mice were randomly divided into five groups, with 10 mice in each group: a clean wound control group(Control group), a seawater immersion injury model group (SII group), a seawater immersion injury with photodynamic therapy group (PDT group), a seawater immersion injury with ciprofloxacin treatment group (CPFX group), and a seawater immersion injury with photodynamic therapy combined with ciprofloxacin treatment group (PDT+CPFX group). The mice in the PDT group underwent a photodynamic therapy of 20 minutes (power density: 15 mW/cm2), the mice in the CPFX group received ciprofloxacin ointment on the wound surface every 24 hours for 7 days, and photodynamic therapy was administered to the PDT+CPFX group for 20 minutes, followed by daily topical application of ciprofloxacin ointment for 7 consecutive days. Wound healing was monitored by photographing the wounds on days 0, 3, 7, 10, and 14 of the experiment. Hematoxylin and Eosin (HE) staining was used to observe the pathological changes of the wounds in each group on days 3, 7, and 10, and to observe whether photodynamic therapy would cause pathological changes in the heart, liver, spleen, lung, kidney and brain tissues of each group of mice. Immunohistochemistry (IHC) was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in in wound of each group of mice on days 3, 7, and 10 of the experiment. Results Compared with the Control group, the SII group showed delayed wound healing, significant inflammatory infiltration, and increased levels of TNF-α and IL-6 (P<0.05). Compared with the SII group, the PDT+CPFX group showed the best wound healing, with significantly better epidermal regeneration and fewer inflammatory cell infiltrates on days 10 and 14 (P<0.05). Photodynamic therapy did not cause significant pathological changes in the heart, liver, spleen, lungs, kidneys or brain of the mice. Conclusions Photodynamic therapy combined with ciprofloxacin synergistically promotes wound healing in seawater immersion injuries.
关键词
海水浸泡伤 /
感染 /
光动力治疗 /
创面愈合 /
炎症反应
Key words
seawater immersion injury /
infection /
photodynamic therapy /
wound healing /
inflammation response
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基金
天津市自然科学基金(23JCQNJC01590);天津市医学重点学科建设(TJYXZDXK-3-001D);后勤学院2025年度科研创新团队项目
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