GLI1在乳腺癌各分子亚型中的表达及意义

PDF(712 KB)

武警医学 ›› 2012, Vol. 23 ›› Issue (10) : 862-865.

论著

GLI1在乳腺癌各分子亚型中的表达及意义

吕遐晟^¹,杜哲明^²,曾朋^²,李良^¹,胡薇^³

作者信息 +

Expression and significance of Gli1 in molecular subtypes of breast cancer

LV Xiasheng^¹,DU Zheming^²,ZENG Peng^²,LI Liang^¹,and HU Wei^³. ¹. Medical Department,². the First Department of Surgery,Jiangxi Provincial Corps Hospital of Armed Police Forces,Nanchang ³³⁰⁰³⁰,China; ³. Department of Breast Surgery,Changhai Hospital,SMMU,Shanghai ²⁰⁰⁴³³,China

Author information +

文章历史 +

摘要

目的探讨Hedgehog信号通路的下游转录因子Gli1与乳腺癌分子标志物的关系及Gli1在乳腺癌各分子亚型中的表达和意义。方法应用免疫组化Envison法检测90例乳腺癌石蜡包埋标本中Gli1的表达，结合标本ERα、Her-2的表达情况，分析Gli1与上述分子标志物的关系及GLI1在分子亚型中表达的差异及意义。结果根据Gli1、ERα、Her-2的不同表达，90例乳腺癌标本中, Gli1阳性38例，ERα阳性60例，Her-2阳性23例。Gli1的表达与ER表达成负相关性，与Her-2表达无明显相关性。各分子亚型标本Gli1阳性率为Basal-like型（66.7%），Her-2过表达型（55.6%），luminal B型(35.7%)，luminal A型(30.4%)。但luminal A型、luminal B型与Her-2过表达型之间，luminal B型、Her-2过表达型与Basal-like型之间Gli1表达差异无统计学意义（P>0.05）。Basal-like型Gli1阳性率高于luminal A型，差异有统计学意义（P<0.05）。结论 Gli1的过表达与乳癌雌激素非依赖性增殖有关，将成为ER阴性乳癌新的治疗靶点。部分basal-like型乳癌增殖可能与HH/Gli1通路激活有关，分子分型法中有必要加入Gli1指标，细化个体化治疗方案及预后判断。

Abstract

Objective To study the relationship between the Hedgehog signaling pathways’ transcription factor Gli1 and molecular markers of breast cancer and to probe its expression and significance in molecular subtypes of breast cancer. Methods Envison immunohistochemical method was used to detect the expression of Gli1 in 90 samples of breast cancer. The relationship between Gli1 and the expression of ER, HER-2 was analyzed. The expression of Gli1 in different molecular subtypes and its significance were discussed. Results The expression of Gli1 and ER were found to be negatively correlated. No significant correlation between Gli1 and HER-2 was exhibited. There was no difference between luminal A, luminal B and HER2 overexpression or between luminal B, HER-2 overexpression and basal-like subtypes (P>0.05). The positive rate of Glil in the basal-like subtype was higher than in luminal A subtype; the difference was statistically significant (P

关键词

乳腺癌 / 分子分型 / Hedgehog信号通路

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

吕遐晟,杜哲明,曾朋,李良,胡薇. GLI1在乳腺癌各分子亚型中的表达及意义[J]. 武警医学. 2012, 23(10): 862-865

LV Xiasheng,DU Zheming,ZENG Peng,LI Liang,and HU Wei. . Medical Department,. the First Department of Surgery,Jiangxi Provincial Corps Hospital of Armed Police Forces,Nanchang,China; . Department of Breast Surgery,Changhai Hospital,SMMU,Shanghai,China. Expression and significance of Gli1 in molecular subtypes of breast cancer[J]. Medical Journal of the Chinese People Armed Police Forces. 2012, 23(10): 862-865

中图分类号： R392.11 R737.9

参考文献

［1］邵志敏，余科达. 乳腺外科发展趋势［J］.中国普外基础与临床杂志，2007，14（3）：252-254.

［2］ Perou C M, Sorlie T, Eisen M B, et al. Molecular portraits of human breast tumours［J］. Nature, 2000, 406(67): 747-752.

［3］ Berman D M, Karhadkar S S, Maitra A，et al. Widespread requirement for Hedgehog ligand Stimulation in growth of digestive tract tumours［J］. Nature, 2003, 425(69): 846-851.

［4］ Feldman I,Feldman G M, Mobarak C, et al. Identification of proteins within the nuclear factor-kappa B transcriptional complex including estrogen receptor-alpha［J］. Am J Obstet Gynecol, 2007,196:394.e1-11.

［5］ Zhou Y,Eppenberger-Castori S,Eppenberger U, et al.The NFkappaB pathway and endocrine-resistant breast cancer［J］.Endocr Relat Cancer,2005,12(Suppl 1):S37-S46.

［6］ Naksshatri H,Bhat-Nakshatri P, Martin D A, et al.Constitutive activation of NF-kappaB during progression of breast cancer to hormone-independent growth［J］.Mol Cell Biol,1997,17:3629-3639.

［7］ Nielsen T O,Hsu F D,Jensen K, et al.Immunohistochemical and clinical characterization of the basal-like subtype of invasive breast carcinoma［J］. Clin Cancer Res，2004,10(16):5367-5374.

［8］ Yu K, Lee C H, Tan P H, et al. Conservation of breast cancer moleullar subtypes and stranscriptional patterns of tumor progression across distinct ethnic populations［J］. Clin Cancer Res, 2004,10(16):5508-5517.

［9］ Carey L A, Perou C M, Livasy C A, et al. Race, breast cancer subtypes, and survival in the Carolina Breast Cancer Study［J］. JAMA, 2006, 295(21): 2492-2502.

［10］ Kubo M, Nakamura M, Tasaki A, et al. Hedgehog signaling pathway is a new therapeutic target for patients with breast cancer［J］. Cancer Res, 2004, 64(17):6071-6074.

［11］崔巍. Sonic hedgehog在乳腺癌中的表达及其表达变化的调节机制［D］. 沈阳：中国医科大学, 2009.

［12］ Zhao J, Chen G, Cao D, et al. Expression of Gli1 correlates with the transition of breast cancer cells to estrogen-independent growth［J］. Breast Cancer Res Treat, 2010, 119(1): 39-51.

［13］赵洁莹, 陈欢, 王雁, 等. 雌激素受体α在乳腺癌细胞中对Gli1转录活性和表达的影响［J］. 第二军医大学学报, 2009, 30(11): 1221-1224.

［14］ Ihemelandu C U, Leffall L D, Dewitty R L, et al. Molecular breast cancer subtypes in premenopausal African-American women, tumor biologic factors and clinical outcome［J］. Ann Surg Oncol, 2007, 14(10): 2994-3003.

［15］ Bauer K R, Brown M, Cress R D, et al. Descriptive analysis of estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and HER2-negative invasive breast cancer, the so-called triple-negative phenotype: a population-based study from the California cancer Registry［J］. Cancer, 2007, 109(9): 1721-1728.

［16］ Smith I, Procter M, Gelber R D, et al. 2-year follow-up of trastuzumab after adjuvant chemotherapy in HER2-positive breast cancer: a randomised controlled trial［J］. Lancet, 2007, 369(9555): 29-36.

［17］ Piccart-Gebhart M J, Procter M, Leyland-Jones B, et al. Trastuzumab after adjuvant chemotherapy in HER2-positive breast cancer［J］. N Engl J Med, 2005, 353(16): 1659-1672.