Effect of DBC1 on biological behavior of liver cancer
GU Yong,DUAN Wei,MENG Hongtao,and WU Xiuhua. Department of Digestive Diseases,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Forces,Xi’an710054,China
Author information+
Show less
文章历史+
收稿日期
修回日期
出版日期
2012-03-27
2012-10-30
2012-11-01
发布日期
2012-10-30
摘要
目的 探讨乳腺癌缺失基因1(deleted in breast cancer 1,DBC1)对肝癌细胞增殖和凋亡的影响。方法 构建pcDNA3.1-DBC1的正义表达载体，转染肝癌细胞SMMC-7721，MTT法检测DBC1对肝癌细胞SMMC-7721生长增殖的影响，采用流式细胞术(FCM)检测DBC1对肝癌细胞SMMC-7721的细胞周期和细胞凋亡的影响。结果 与转染空载体的SMMC-7721细胞相比，pcDNA3.1-DBC1正义表达载体转染上调DBC1的表达，可显著抑制肝癌细胞（SMMC-7721）增殖，升高G1期细胞比例（51.0% vs 64.9%，P=0.002），降低S期细胞比例（29.7% vs 14.0%， P<0.001），诱导细胞凋亡（8.8% vs 24.6%， P<0.001）。结论 DBC1通过抑制肿瘤细胞增殖，阻滞细胞周期进展，促进细胞凋亡，抑制肝癌的发生和发展。
Abstract
Objective To study the effect of deleted genes of breast cancer 1(DBC1) onproliferation and apoptosis in liver cancer cells. Methods pcDNA3.1-DBC1 vector was constructed and transfected into human liver cancer cell line SMMC-7721. MTT assay and flow cytometry were performed to detect the effect of DBC1 on the proliferation, cycle, and apoptosis of cells. Results Compared with cells transfected with the control vector, entropic expression of DBC1 gene with pcDNA3.1-DBC1 vector significantly inhibited SMMC-7721 proliferation. Entropic expression of DBC1 caused a significant increase in the percentage of cells in the G1 phase （51.0% vs 64.9%, P=0.002），a significant decrease in the percentage of cells in the S phase （29.7% vs 14.0%, P
GU Yong,DUAN Wei,MENG Hongtao,and WU Xiuhua. Department of Digestive Diseases,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Forces,Xi’an,China.
Effect of DBC1 on biological behavior of liver cancer[J]. Medical Journal of the Chinese People Armed Police Forces. 2012, 23(10): 871-874
中图分类号：
R735.7
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］ Hamaguchi M,Meth J L,von Klitzing C,et al. DBC2, a candidate for a tumor suppressor gene involved in breast cancer［J］. Proc Natl Acad Sci U S A ,2002,99(21):13647-13652.
［2］ Lee H,Kim K R,Noh S J,et al. Expression of DBC1 and SIRT1 is associated with poor prognosis for breast carcinoma［J］. Hum Pathol, 2011,42(2): 204-213.
［3］ Cha E J,Noh S J,Kwon K S,et al. Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma［J］. Clin Cancer Res,2009,15(13): 4453-4459.
［4］ Kim S H,Kim J H,Yu E J,et al. The over-expression of DBC1 in esophageal squamous cell carcinoma correlates with poor prognosis［J］. Histol Histopathol,2012,27(1): 49-58.
［5］ Kamangar F,Dores G M,Anderson W F. Patterns of cancer incidence,mortality,and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world［J］. J Clin Oncol,2006,24(14): 2137-2150.
［6］ Kim J E,Chen J,Lou Z. p30 DBC is a potential regulator of tumorigenesis［J］. Cell Cycle, 2009,8(18):2932-2935.
［7］ Fu J,Jiang J,Li J ,et al. Deleted in breast cancer 1,a novel androgen receptor (AR) coactivator that promotes AR DNA-binding activity［J］. J Biol Chem,2009,284(11):6832-6840.
［8］ Kim J E,Chen J,Lou Z. DBC1 is a negative regulator of SIRT1［J］. Nature,2008, 451(7178):583-586.
［9］ Koch H B,Zhang R,Verdoodt B,et al. Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach［J］. Cell Cycle,2007,6(2):205-217.
［10］ Sundararajan R,Chen G,Mukherjee C,et al. Caspase-dependent processing activates the proapoptotic activity of deleted in breast cancer-1 during tumor necrosis factor alpha mediated death signaling［J］. Oncogene,2005,24(31):4908-4920.
［11］ Hanahan D,Weinberg R A. Hallmarks of cancer: the next generation［J］. Cell,2011,144(5):646-674.
［12］ Murray A W. Recycling the cell cycle: cyclins revisited［J］. Cell,2004,116(2): 221-234.
［13］ Muntané J. Targeting cell death and survival receptors in hepatocellular carcinoma［J］. Anticancer Agents Med Chem,2011,11(6):576-584.
［14］ Kim J E,Sung S. Deleted in breast cancer 1 (DBC1) is a dynamically regulated protein［J］. Neoplasma,2010,57(4):365-368.
［15］ Trauernicht A M,Kim S J,Kim N H, et al. DBC-1 mediates endocrine resistant breast cancercell survival［J］. Cell Cycle,2010,9(6):1218-1219.
［16］ Hiraike H,Wada-Hiraike O,Nakagawa S,et al. Identification of DBC1 as a transcriptional repressor for BRCA1［J］. Br J Cancer,2010,102(6):1061-1067.
PDF(879 KB)
