目的 检测IL-23在鼻息肉(nasal polyp ,NP)组织中的表达，探讨其在鼻息肉发病中的作用。方法 收集15例鼻息肉标本（NP组）和15例在鼻中隔偏曲矫正手术中根据需要部分切除下鼻甲黏膜组织(对照组)，分别采用免疫组织化学方法和RT-PCR方法检测两组IL-23蛋白和IL-23p19mRNA表达水平。结果 NP组IL-23蛋白阳性表达炎性浸润细胞数明显多于对照组，单位目标面积光密度均值 (0.321±0.023)明显高于对照组(0.199±0.011)，差异有统计学意义(P＜0.01)；IL-23p19mRNA表达水平 (0.676±0.077)明显高于对照组(0.212±0.015)，差异有统计学意义(P＜0.01)。结论 IL-23可能在参与了NP发病过程。
Abstract
Objective To investigate the expression and role of interleukin-23(IL-23) in nasal polyp patients. Methods NP tissues of 15 nasal polyp(NP) patients and 15 simple deviations of normal inferior turbinate tissues of nasal septum patients were collected. mRNA and protein expression of IL-23 were detected by means of immunohistochemistry and RT-PCR. Results Immunohistochemistry staining demonstrated that there were far more IL-23 positive cells in NP patients than in normal controls. The average expression level of IL-23 protein in NP tissues (0.321±0.023) was significantly different (P<0.01) from that of normal inferior turbinate tissues (0.199±0.011). RT-PCR demonstrated that the average expression level of IL-23p19mRNA in NP tissues (0.676±0.077) was significantly different (P＜0.01) from that in normal inferior turbinate tissues(0.212±0.015). Conclusions IL-23 may be involved in the chronic inflammation of NP.
关键词
白细胞介素23 /
鼻息肉 /
免疫组化 /
逆转录聚合酶链反应
Key words
interleukin-23 /
nasal polyp /
immunohistochemistry /
RT-PCR
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