目的 观察白藜芦醇( resveratrol，Res) 对人膀胱癌细胞T24 生长增殖的影响，初步探讨miR-34a 在Res影响T24 细胞中的作用。方法 不同浓度Res分别作用T24细胞24 h，采用甲基噻唑基四唑（methyl thiazolyl tetrazolium, MTT）法检测细胞增殖，流式细胞术检测细胞凋亡，Western blotting检测p53蛋白水平，实时定量PCR（Real-time PCR）检测T24 细胞miR-34a 的基因表达。结果 Res剂量依赖性地抑制T24细胞增殖，诱导细胞凋亡，明显增加p53的蛋白表达。此外，Res处理后T24 细胞中miR-34a 的基因表达亦显著升高。结论 Res能够有效抑制膀胱癌细胞生长增殖，可能与诱导细胞凋亡、上调miR-34a及p53表达有关。
Abstract
Objective To investigate the effects of resveratrol on proliferation of human bladder cancer cell line T24，and the role of miR-34a in its mechanism. Methods T24 cells were treated with resveratrol in different concentrations for 24 h. Cell proliferation was validated by MTT. Flow cytometry was used to analyse apoptosis. The expression of miR-34a was detect by real-time PCR. Results Resveratrol could inhibit the proliferation of T24 in a dose dependent manner, induce apoptosis and increase the expression of p53 significantly. Furthermore, the level of miR-34a in T24 cells was higher in resveratrol-treated group than that in the control group. Conclusions Resveratrol can suppress the proliferation of human bladder cancer cells, which may be attributed to inducing apoptosis, up-regulating miR-34a and p53.
关键词
白藜芦醇 /
膀胱癌 /
细胞凋亡 /
miR-34a /
p53
Key words
resveratrol /
bladder cancer /
apoptosis /
miR-34a /
p53
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参考文献
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(2012-05-19
基金
武警后勤学院附属医院种子基金面上项目（FYM201211），武警后勤学院科研基金面上项目（WYM201016）
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