目的 探讨泌尿系感染病原菌的分布及耐药性,为合理应用抗菌药物提供依据。 方法 采用美国Walkaway96全自动微生物分析仪进行微生物鉴定和最低抑菌浓度MIC测定。 结果 1095份中段尿标本中，分离出病原菌246株，阳性率为22.5%。以革兰阴性菌为主，尤以大肠埃希菌最常见；革兰阳性菌以粪肠球菌和屎肠球菌为主；真菌感染的数量亦明显增加；药敏试验发现1例耐万古霉素的屎肠球菌；产ESBLs的大肠埃希菌、肺炎克雷伯菌和奇异变形杆菌的检出率分别为51.4%、47.1%和16.7%。肠杆菌科细菌对亚胺培南敏感率均为100.0%,对其他抗菌药物产生了不同程度的耐药性。 结论 及时总结分析泌尿系感染病原菌的分布及耐药性，对于控制耐药菌株传播、指导合理选用抗菌药物具有十分重要的意义。

Objective To investigate the distribution and resistance of pathgenic bacteria in urinary tract infection and offer scientific basis for proper use of antibiotics.  Method Species identification and antibiotics susceptibility test were performed by Walkway 96 automicroscan systems(America).  Results A total of 246 strains of bacteria were isolated from  urine samples , most of which were Gram-negative bacteria with Escherichia coli as the most common. Enterococci were the most common Gram-positive bacteria, especially for E. faecalis and E. faecium. Fungal infection also increased. There was one case of vancomycin-resistant Enterococc (VRE) in invro antibiotics susceptibility test. The detection rate of ESBLs producing E. coli, Klebsiella penumoniae and Proteus was 51.4%, 47.1% and 16.7%, respectively. The susceptiblity rate of entrobacteriaceae to imipenem was 100.0%.  Conclusions It is  of great significance to analyze the distribution and antibiotic resistance of isolated bacteria in urine tract infection to control resistant strains.