目的 了解住院患者感染的金黄色葡萄球菌（methicillin sensitive staphylococcus aureus，MSSA）对常用抗生素的耐药情况，以指导临床合理使用抗生素。 方法 应用VITEK32微生物自动鉴定分析系统，采用K-B法对2009-03至2011-10临床各科室分离的158株MSSA进行药物敏感性试验，并对其药敏结果进行统计分析。 结果 158株MSSA中耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus，MRSA）96株，占60.76%，对青霉素G、氨苄西林100%耐药，对万古霉素100%敏感；对甲氧西林敏感MSSA,占39.24%，对青霉素G、氨苄西林耐药率分别为69.35%和67.74%，对利奈唑胺、喹努普汀/达福普汀和万古霉素100%敏感。 结论 MSSA是临床感染的主要病原菌之一，分析MSSA耐药情况，可以指导临床合理选用抗生素。

 Objective  To study the resistance of clinically-isolated  Staphylococcus aureus  to antimicrobial agents so as to facilitate the rational use of antibiotics.  Methods  The pathogenic bacteria were evaluated by VITEK32. Drug susceptibility test was carried out by K-B method. The results of 158 strains of  Staphylococcus aureus  were analysed by WHONET.  Results  96 strains of MRSA were detected from 158 strains of  Staphylococcus aureus ,which accounted for 60.76%. All the MRSA was resistant to penicillin and ampicillin but was sensitive to vancomycin. The resistance rate of MSSA to penicillin and ampicillin was 69.35% and 67.74%, respectively, and all the MSSA was sensitive to linezolid, quinupristin/dalfopristin and vancomycin.  Conclusions   Staphylococcus aureus  is one of the main pathogens of clinical infection. Analysis of drug resistance of  Staphylococcus aureus  can guide the clinical use of antibiotics.