目的 探讨人β防御素2(human beta defensin 2, hBD-2)基因作用的分子机制，为临床抗感染治疗提供依据。方法 肺炎军团杆菌感染NCI-H292细胞，采用菌落形成实验，Western blot实验和ELISA实验检测丝裂原活化蛋白激酶（mitogen-activatived protein kinase,MAPK）途径内基因的变化。结果 hBD-2对肺炎军团杆菌有抑制作用，肺炎军团杆菌诱导分泌hBD-2主要是通过JNK和p38途径起作用。结论 肺炎军团杆菌主要通过c-Jun氨基末端激酶(the c-Jun N-terminal kinase,JNK)和p38途径诱导NCI-H292细胞分泌hBD-2。
Abstract
Objective To investigate the role of hBD-2 gene for the clinical anti-infective therapy. Methods Legionella pneumoniae infection in NCI-H292 cells, using colony formation assay, Western blotting , and ELISA to investgate the related gene changes in the MAPK pathway. Results hBD-2 inhibited Legionella pneumoniae,and Legionella pneumoniae induced secretion of hBD-2 mainly through the JNK and p38 pathway. Conclusions Legionella pneumoniae induces hBD-2 secreted from NCI-H292 cells,which is mainly due to JNK and p38 pathway.
关键词
军团杆菌 /
人β防御素2 /
丝裂原活化蛋白激酶
Key words
legionella pneumoniae /
human beta defensin 2 /
mitogen-activatived protein kinase
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