目的 探讨CA153、细胞角蛋白19片段(CYFRA21-1)、神经特异性烯醇化酶(NSE)联合检测在肺肿瘤鉴别诊断中的临床价值。方法 88例非小细胞肺癌，31例小细胞肺癌，94例肺部良性疾病和92例健康人入选本研究，应用Cobras e601全自动电化学发光免疫分析仪检测肿瘤标志物，比较各组CA153、CYFRA21-1、NSE水平。结果 非小细胞肺癌组CA153、CYFRA21-1水平高于小细胞肺癌组、良性病变组和健康对照组(P<0.05)。而小细胞肺癌组NSE水平高于其他三组(P<0.05)。单一肿瘤标志物检测诊断非小细胞肺癌，CYFRA21-1的敏感性最高，NSE的特异性最高;3种肿瘤标志物联合应用可提高非小细胞肺癌诊断的敏感性和特异性。结论 CA153、CYFRA21-1、NSE的联合检测对非小细胞肺癌的诊断具有较大的价值。

Objective To study the value of combined cytokeratin fragment 19(CYFRA21-1) and neuron-specific enolase(NSE). Methods Tumor markers were detected in 88 patients with none-small cell lung cancer(USCLC),31 patients with small cell lung cancer(SCLC),94 patients with benign lung diseases as well as 92 healthy controls using Cobras e601 electrochemical luminescence immunoassay techniques and automatic biochemical analyzer. Results The CA153 and CYFRA21-1 levels were significantly higher in patients with USCLC than in those with SCLC and in benign disease and healthy groups(P<0.05).Furthermore,in the SCLC group,the NSE level was higher than that in other groups(P<0.05).The sensitivity of single CYFRA21-1 was higher in diagnosis of USCLC and so was the specificity of single NSE in diagnosis.The sensitivity of combined CA153,CYFRA21-1 and NSE were higher. Conclusions Combined CA153,CYFRA21-1 and NSE test can increase its sensitivity and accuracy in diagnosis of USCLC,thus having a greater value in its diagnosis.