目的 探讨应力作用下鼠成骨细胞蛋白质表达谱的变化。方法 将鼠成骨细胞分为加载组和对照组， 采用四点弯曲细胞力学加载装置， 以频率为0.5 Hz， 应变水平2000 με条件下加载6 h后， 运用双向凝胶电泳分离蛋白样品、分析差异表达蛋白点。结果 加载组和对照组分别得到( 533±14)和( 512±11)个蛋白点， 其中有9种蛋白质表达出现明显差异， 7种在应变作用后表达增高， 2种降低。结论 应力作用下鼠成骨细胞的蛋白表达发生了显著变化， 这些差异蛋白可能参与成骨细胞力学反应机制的不同过程。

Objective To study proteomic differential expressions of mouse osteoblasts stimulated by mechanical strain. Methods Mouse osteoblasts were randomly divided into two groups:experimental group were subjected to mechanical strain of 0.5 Hz, 2000 με for 6 hours by using four-point-bend loading system;control group did not undergo any stress. Proteins extracted from cells were separated by two-dimensional electrophoresis ( 2-DE), and the differentially expressed protein spots were analysed. Results A total of( 533±14)and( 512±11) protein spots were resolved by 2-DE of experimental and control groups extractions , respectively.Nine protein points displayed significant changes in strain treated osteoblasts, 7 increased in expression and 2 decreased. Conclusions The osteoblasts can express differential proteins stimulated by mechanical strains and these proteins may play an important role in molecular mechanism of osteoblasts under mechanical strain loading.