目的 研究鲑鱼降钙素（salmon calcitonin, sCT）对前交叉韧带切断（anterior cruciate ligament transection, ACLT）模型兔膝关节软骨损伤的影响。方法 将48只健康3月龄雄性日本大耳白兔随机分为3组，每组16只。选择其中两组造模，术后分别给予sCT和生理盐水（normal saline, NS），即ACLT+sCT组和ACLT+NS组；另外一组作为对照组，不予任何处理。8周后处死动物，取关节软骨进行HE染色观察并进行Mankin评分，免疫组化方法检测软骨中MMP-13表达情况；采用骨组织形态计量学比较各组软骨下骨小梁结构变化。结果 软骨HE染色观察、Mankin评分及MMP-13组化评价结果表明，ACLT+sCT组软骨损伤程度轻于ACLT+NS组，但较对照组严重，各组间比较差异有统计学意义（P＜0.05）。软骨下骨小梁结构定量分析表明，ACLT+sCT组软骨下骨小梁厚度明显高于ACLT+NS组［（263.56±24.30）vs（182.63±182.63）］μm，但低于对照组（361.49±23.36）μm，各组间比较差异具有统计学意义（P＜0.05）。结论 sCT能够降低ACLT切断所造成的兔膝关节软骨组织损伤程度，同时可部分维持软骨下骨小梁结构，对骨性关节炎软骨有一定保护作用。
Abstract
Objective To investigate the effects of salmon calcitonin on knee joint cartilage lesions in anterior cruciate ligament transection (ACLT) rat models. Methods Forty-eight three-month-old male rabbits were randomly divided into three groups(n=16). Two groups of rabbits underwent anterior cruciate ligament transection（ACLT）surgery, then were subcutaneously injected salmon calcitoin(sCT) and normal saline separately. The other rabbit were untreated as control. After eight weeks, all the rabbits were killed. The sections of cartilage were stained with hematoxylin-eosin (HE), and analyzed by Mankin score system. Expression levels of MMP-13 were detected by immunohistochemistry staining. Subchondral bone of proximal tibia was measured by bone histomorphometic method. Results Based on the observation of HE staining, Mankin score and the expression of MMP-13, the articular cartilage lesions in ACLT+sCT group were less than that in ACLT+NS group(P＜0.05), but more severe than that in control group(P＜0.05). Subchondral bone histomorphometic analysis indicated that the data in trabecular number and trabecular thickness in ACLT+sCT group was higher than those in ACLT+NS group (P＜0.05), but lower than those in control group(P＜0.05); The data in trabecular separation in ACLT+sCT group was lower than that in ACLT+NS group(P＜0.05), but higher than that in control group(P＜0.05). Conclusions sCT reduces the cartilage lesions and maintains subchondral trabecular structure in ACLT rabbit models. sCT exhibits partly chondroprotection in experimental osteoarthritis.
关键词
鲑鱼降钙素 /
前交叉韧带切断 /
软骨损伤
Key words
salmon calcitonin /
anterior cruciate ligament transection /
cartilage lesions
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(2012-10-16
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