目的 探讨肌红蛋白（myoglobin, Mb）基因第2外显子单核苷酸多态（A79G）与运动性肌肉损伤的关系。方法 85名武警战士进行一次大强度负重运动，检测血清肌酸激酶（creatine kinase，CK）活性的时程变化；聚合酶链反应-限制性片段长度多态（PCR-RFLP）法测定Mb基因多态性；分析基因多态性与安静CK（CKpre）、峰值CK（CKpeak）及CK最大变化值（△CK）的关系。结果 CKpre为（145±33）U/L，大强度运动后24 h开始逐渐升高，72 h达峰值［CKpeak：（2972±1648）］U/L，随后缓慢下降，120 h仍高于安静水平。3种基因型的分布频率分别为AA（54.1%）、AG（40.0%）、GG（5.9%），等位基因分布频率为A（74.1%）、G（25.9%），符合Hardy-Weinberg遗传平衡定律。组间比较，（AG+GG）组CKpre、CKpeak和△CK均高于AA组（均P＜0.01）。结论 运动性肌肉损伤易感性与Mb基因第2外显子单核苷酸多态（A79G）有关，G等位基因具有肌肉损伤易感性，而AA基因型则具有运动性肌肉损伤保护作用。
Abstract
Objective To study the relationship between single nucleotide polymorphism (A79G) and (T109C) in the second exon of the myoglobin gene and exercise-induced muscle damage. Methods Eighty-five soldiers of CAPF performed a strenuous weight-loading exercise and the time course of serum creatine kinase (CK) activity was measured. Myoglobin gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Association between resting CK (CKpre), maximal CK (CKpeak), maxiaml changes in CK (△CK) and gene polymorphism was then analysed. Results The activity of CK ［CKpre: (145±33) U/L］ gradually increased at 24 h after strenuous exercise and reached peak value at 72 h［CKpeak: (2972±1648) U/L］, which then slowly reduced, but still higher than rest level at 120 h. The genotype frequencies (AA=54.1%, AG=40.0%, GG=5.9%) and allele frequencies (A=74.1%, G=25.9%) were coincident with Hardy-Weinberg equilibrium. Compared between groups, CKpre, CKpeak and △CK in (AG+GG)-group were all higher than those in AA genotype group (all P＜0.01). Conclusions The single nucleotide polymorphism (A79G) in the second exon of the myoglobin gene might contribute to susceptivity of exercise-induced muscle damage. Subjects with G allele have increased risk for developing muscle damage, whereas AA genotype may have protective effects.
关键词
肌红蛋白 /
基因多态 /
运动性肌肉损伤 /
肌酸激酶 /
亚临床运动性横纹肌溶解症
Key words
myoglobin /
gene polymorphism /
exercise-induced muscle damage /
creatine kinase /
subclinical exertcise rhabdomyolysis
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］ Kenney K, Landau M E, Gonzalez R S, et al. Serum creatine kinase after exercise: drawing the line between physiological response and exertional rhabdomyolysis［J］. Muscle Nerve, 2012,45(3):356-362.
［2］ Heled Y, Bloom M S, Wu T J, et al. CK-MM and ACE genotypes and physiological prediction of the creatine kinase response to exercise［J］. J Appl Physiol, 2007,103(2):504-510.
［3］ Alpers J P, Jones L K Jr. Natural history of exertional rhabdomyolysis: a population-based analysis［J］. Muscle Nerve，2010，42(4): 487-91.
［4］ Galvez R, Stacy J, Howley A. Exertional rhabdomyolysis in seven division-1 swimming athletes［J］. Clin J Sport Med, 2008,18(4):366-368.
［5］ Landau M E, Kenney K, Deuster P, et al. Exertional rhabdomyolysis: a clinical review with a focus on genetic influences［J］. J Clin Neuromuscul Dis, 2012,13(3):122-136.
［6］ Johlig L, Valberg S J, Mickelson J R, et al. Epidemiological and genetic study of exertional rhabdomyolysis in a Warmblood horse family in Switzerland［J］. Equine Vet J, 2011,43(2):240-245.
［7］ 刘 杰, 骆 媛, 钱远宇, 等. 运动性横纹肌溶解高反应者ACE/CK-MM基因多态性分析［J］. 山东医药, 2009,49(26):21-23.
［8］ Devaney J M, Hoffman E P, Gordish-Dressman H, et al. IGF-II gene region polymorphisms related to exertional muscle damage［J］. J Appl Physiol, 2007,102(5):1815-1823.
［9］ 杨贤罡, 李燕春, 胡 扬. ACTN3基因R577X多态性与运动能力的关联性研究:Meta分析［J］. 体育科学, 2011,31(3):44-52.
［10］ Yamin C, Duarte J A, Oliveira J M, et al. IL6 (-174) and TNFA (-308) promoter polymorphisms are associated with systemic creatine kinase response to eccentric exercise［J］. Eur J Appl Physiol, 2008,104(3):579-586.
［11］ Hubal M J, Devaney J M, Hoffman E P, et al. CCL2 and CCR2 polymorphisms are associated with markers of exercise-induced skeletal muscle damage［J］. J Appl Physiol, 2010,108(6):1651-1658.
［12］ 吴 剑, 胡 扬, 包大鹏. 中国北方汉族人群肌红蛋白基因第2外显子多态性的研究［J］. 首都体育学院学报, 2009,21(3):346-348.
［13］ 吴 剑, 胡 扬, 刘 刚, 等. 肌红蛋白基因多态与有氧训练效果的关联性分析［J］. 中国运动医学杂志, 2005,24(6):650-654.
(2012-09-17
基金
武警后勤学院博士启动基金（WYB201106）和武警后勤科研项目（WJHQ2012-21）
PDF(761 KB)
