目的 观察0.1 μmol/L白藜芦醇作用不同受体亚型(ERα⁺/ERβ⁺)MCF-7细胞的增殖情况, 并探讨其作用机制。方法 采用MTT法和流式细胞术检测白藜芦醇对细胞增殖及周期的影响；Western-blot法检测白藜芦醇与不同受体亚型结合后对细胞信号通路的影响。结果 白藜芦醇作用后, 细胞增殖活性ERα^-ERβ⁺-MCF-7<MCF-7<ERα⁺ERβ^--MCF-7；同时流式细胞术结果显示, ERα⁺细胞S期比例增加（P<0.05）, 而ERβ⁺细胞周期阻滞于G₀/G₁期。western-blot结果显示, 白藜芦醇可提高ERα⁺细胞bcl-2、ERK-P和AKT-P蛋白表达, 减少caspase-3、p38-P蛋白表达；对ERβ⁺细胞作用则相反。结论 白藜芦醇对MCF-7细胞的双相调节作用可能与ER不同亚型亲和力有关。

Objective To observe the effect of resveratrol proliferation of different estrogen receptor subtypes of MCF-7 cells, and to study the mechanism. Methods MTT assay and flow cytometry were carried out to examine the abilities of cell proliferation. Western blotting method was used to detect the changes of the signal pathway. Results The abilities of cell proliferation were ERα^-ERβ⁺-MCF-7＜MCF-7＜ERα⁺ERβ^--MCF-7. The S-phase of the ERα⁺ cell cycle was increased by resveratrol, however, ERβ⁺ cells was arrested at phase G₀/G₁. The protein expression of bcl-2, ERK-P and AKT-P were increased in ERα⁺ cells treated with RES. The effect of ERβ⁺ cells was opposite. Conclusions The dual regulatory effect on MCF-7 cells by resveratrol may be related to the affinity of different estrogen receptor subtypes.