目的 研究针刺四关、足三里穴对D-半乳糖和亚硝酸钠致阿尔茨海默病（Alzheimer’s disease, AD）模型大鼠学习记忆能力及超氧化物歧化酶（super oxide dismutase, SOD）、丙二醛（malondialdehyde, MDA）的影响。方法 选取SD级雄性大鼠40只，随机分为模型组、对照组、针刺组和空白组各10只。空白组不给予治疗造模，其余各组每天腹腔注射D-半乳糖(120 mg/kg)和亚硝酸钠（90 mg/kg）造模，连续注射6周。造模21 d后针刺组予针刺四关、足三里穴干预治疗,对照组给予盐酸多奈哌齐混悬液(0.45 mg/kg)灌胃治疗。3个疗程后，采用Morris水迷宫测试四组大鼠的学习记忆能力，通过ELISA检测脑组织SOD、MDA的活性。结果 治疗后，针刺组和对照组与模型组大鼠比较平均逃避潜伏的时间减少(P<0.01)；大鼠的穿环次数提高（P<0.05）；针刺组SOD活性为（86.23±2.54） U/mg和对照组脑组织SOD的活性（85.70±4.02） U/mg显著高于模型组（64.97±4.27） U/mg（P<0.01），MDA含量低于模型组（P<0.01）。结论 针刺四关穴、足三里穴可改善大鼠的学习记忆能力，提高SOD活性，减少MDA含量，具有抗氧化，保护神经细胞的作用，从而延缓衰老的发生。

Objective To study the effect of needling Siguan and Zusanli (ST36) on the learning and memory ability, superoxide dismutase (SOD), and malondialdehyde (MDA) of model rats with D-galactose and sodium nitrite-induced Alzheimer’s disease (AD). Methods Forty SD male rats were selected and randomly divided into model group, control group, needling group and blank group (10 rats for each group). Except the blank group, other groups were modeled with intraperitoneal injection of d-galactose (120 mg/kg) and sodium nitrite (90 mg/kg) for a total of 6 weeks in sequence. 21 days after the modelling, the needling group underwent intervention treatment by needling Siguan and Zusanli, and the control group was subjected to lavage treatment of Donepezil suspension (0.45 mg/kg). After three courses, Morris water maze was used to test the learning and memory ability of the four groups, and ELISA was used to test the activity of SOD and MDA in the brain. Results After the treatment, compared with model group, needling group and control group have shorter average escape latency (P<0.01); more crossing times (P<0.05); significantly higher SOD activity (P<0.01) and lower MDA content (P<0.01). Conclusions Needling Siguan and Zusanli can improve the learning and memory ability of rats, increase SOD activity and reduce MDA content, featuring antioxidation and protecting neuron to delay senility.