目的 研究胡黄连提取物对缺氧复氧后肾小管上皮细胞氧化损伤的保护作用。方法 采用肾小管上皮细胞建立缺氧复氧损伤模型（分别缺氧0.5、1、2 h），取缺氧2 h组分别用不同浓度胡黄连提取物（10、100、1000 μg/ml）进行预处理，采用流式细胞术检测不同组细胞凋亡及细胞内氧化应激水平。结果 正常情况下肾小管上皮细胞内氧化应激水平非常低，可见极少量凋亡细胞和死亡细胞，与正常对照组相比，各缺氧复氧组细胞内氧化应激水平提高（P<0.05），凋亡细胞和死亡细胞数量明显增多（P<0.05），且随缺氧时间的延长，细胞内氧化应激水平逐渐升高（P<0.05），凋亡细胞和死亡细胞数量逐渐增多（P<0.05）。与单纯缺氧复氧组相比，不同浓度胡黄连提取物呈剂量关系抑制细胞内氧化应激水平（P<0.05），同时呈剂量关系减少凋亡细胞和坏死细胞的数量（P<0.05）。结论 胡黄连提取物可以通过抑制细胞内氧化应激减少肾小管上皮细胞缺氧复氧后细胞凋亡和坏死。
Abstract
Objective To investigate the mechanism of oxidative stress of renal tubular epithelial cells with hypoxia reoxygenation (H/R) and to evaluate the effects of extracts of Picorhiza scrophlariiflora Pennell on them. Methods The renal epithelial cells were reoxygenated after hypoxia for 0.5h,1h or 2h to set up H/R cell model.The renal epithelial cells were divided into the following groups: normal control grounp,H/R group, 10 μg/ml extracts of Picorhiza scrophlariiflora Pennell+H/R group, and 100 μg/ml extracts of Picorhiza scrophlariiflora Pennell+H/R group, 1000 μg/ml extracts of Picorhiza scrophlariiflora Pennell+H/R group. The flow cytometry was used to detect the oxidative and the apoptosis of renal tubular epithelial cells Result The oxidative stress in renal tubular epithelial cells was low. The amounts of apoptosis and necrosis were small.The oxidative stress in renal tubular epithelial cells was induced and increasd after H/R, compared with that in normal HK2 cells（P<0.05）. The amounts of apoptosis and necrosis were increasd after H/R, compared with that in normal HK2 cells（P<0.05）.Oxidative stress in renal tubular epithelial cells after H/R was inhibited by extracts of Picorhiza scrophlariiflora Pennell in a dose-dependent manner（P<0.05）. The apoptosis and necrosis of renal epithelial cells after H/R were inhibited by extracts of Picorhiza scrophlariiflora Pennell in a dose-dependent manner（P<0.05）. Conclusion Extracts of Picorhiza scrophlariiflora Pennell protect renal epithelial cells against apoptosis and necrosis by inhibiting oxidation after H/R injury.
关键词
肾小管上皮细胞 /
缺氧复氧 /
胡黄连提取物
Key words
renal tubular epithelial cells /
hypoxia reoxygenation /
extracts of picorhiza scrophlariiflora pennell
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