&alpha;晶体蛋白促进大鼠嗅鞘细胞存活的体外研究

王艳华; 王东武; 阴正勤

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武警医学 ›› 2013, Vol. 24 ›› Issue (4) : 316-318.

SARS专题

α晶体蛋白促进大鼠嗅鞘细胞存活的体外研究

王艳华^¹,²,王东武^²,阴正勤^¹

作者信息 +

Alpha-crystallin promotes OECs survival in vitro

WANG Yanhua^¹,²,WANG Dongwu^²,and YIN Zhengqin^¹.

Author information +

文章历史 +

摘要

目的研究α晶体蛋白对离体培养的嗅鞘细胞(olfactory ensheathing cells, OECs )存活和增殖的影响，探索增强移植OECs存活和增殖的有效方法。方法取大鼠嗅球进行OECs离体培养，细胞计数检测α晶体蛋白组及对照组OECs的存活数目；Brdu掺入法及流式细胞仪检测OECs的增殖。结果 (1)培养5 d α晶体蛋白组OECs数目与对照组相比无明显差异，培养6~13 d，α晶体蛋白组OECs数目明显多于对照组（P<0.05）；（2）将Brdu加入培养液中8 h后，α晶体蛋白组同视野Brdu阳性细胞数与总细胞数的百分比显著大于对照组（P<0.01）；（3）α晶体蛋白组G₂/M期、S期细胞数明显多于对照组（P<0.01）。结论 α晶体蛋白能明显促进离体培养的OECs的存活。

Abstract

Objective To investigate the effect of α-crystallin on the survival and proliferation of olfactory ensheating cells(OECs) in vitro. Methods OECs were cultured and treated with α-crystallin in DMEM/F12 or DMEM/F12 only. The number of surviving OECs was counte for after cultured 5days. The number of Brdu-labeled OECs was counted after Brdu was added in DMEM/F12. And cell cycle was analyzed by flow cytometry. Results α-crystallin promoted the survival of OECs at 6-13 days. The Brdu-labeled OECs in α-crystallin group was more in number than in control group. And the cell percentage of G₂/M state and S state was higher in α-crystallin group than in control group. Conclusions α-crystallin promotes OECs survival and proliferation.

导出引用

王艳华,王东武,阴正勤. α晶体蛋白促进大鼠嗅鞘细胞存活的体外研究[J]. 武警医学. 2013, 24(4): 316-318

WANG Yanhua,WANG Dongwu,and YIN Zhengqin.. Alpha-crystallin promotes OECs survival in vitro[J]. Medical Journal of the Chinese People Armed Police Forces. 2013, 24(4): 316-318

中图分类号： R776

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