目的 观察脓毒症患者血清中miRNA-122的表达量在病程中的动态变化情况,并探讨其与脓毒症严重程度的关系。方法 选择52例脓毒症患者,其中一般脓毒症23例,重症脓毒症29例,23例健康人作为对照。采用荧光定量 PCR(qRT-PCR) 检测研究对象在入组第1,3,5,7,10,14天血清中 miRNA- 122表达量,并记录患者的基本临床信息。结果 在脓毒症患者入组后14 d的病程中,52例脓毒症患者和23例健康人群血清中的miRNA-122表达量在每个时间点比较,前者均比后者显著下降(P＜0.01)。用入组第1天的miRNA表达量做ROC诊断分析得出,miRNA-122诊断脓毒症的特异性是95.7%,敏感性是53.8%。重症脓毒症组的血清miRNA-122表达量水平高于一般脓毒症组,并且在各个观测时间点两组表达量具有统计学差异(P＜0.01)。结论 血清中miRNA-122可能作为脓毒症诊断的特异性标志物,具有潜在的临床价值,并且miRNA-122在预警脓毒症患者疾病严重程度方面具有积极的临床意义。
Abstract
Objective To study the dynamic changes in serum miRNA-122 levels during sepsis and the correlation between serum miRNA-122 and sepsis severity. Methods Fifty-two sepsis patients (including 23 non-severe septic patients and 29 severe septic patients)and 23 healthy controls were recruited in the study. The expression levels of serum miRNA-122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays (qRT-PCR).Levels of serum miRNA-122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients clinical information was also recorded. Results After comparison, levels of serum miRNA-122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls (P＜0.01). The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA-122 on the first day of their admissions. When the cut-off point was set at 0.710, miRNA-122 had a specificity of 95.7% and a sensitivity of 53.8%. The expression levels of serum miRNA-122 in severe sepsis group were significantly higher than in sepsis group on each observation time point (P＜0.01). Conclusions Serum miRNA-122 can used as a specific biomarker for diagnosis of sepsis. And miRNA-122 might be correlated with the organ injuries of septic patients.
关键词
脓毒症 /
微小RNA-122 /
微小RNA /
诊断
Key words
sepsis /
microRNA-122 /
microRNA /
diagnosis
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参考文献
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基金
国家自然科学基金(81170008);军队十二五面上课题(CWS11J094)
