目的探讨PCR-荧光探针法与抗-HCV检测在丙型肝炎(丙肝)诊断中的应用价值。方法回顾性分析265例疑似丙肝病毒感染者行HCV-RNA、抗-HCV及ALT检测的资料,统计不同年龄、性别分布情况,进行HCV-RNA与抗-HCV、ALT的相关性分析。结果(1)男性抗-HCV与HCV-RNA的阳性率均高于女性,但差异无统计学意义(P>0.05);年龄>40岁的患者抗-HCV与HCV-RNA的阳性率明显高于年龄≤40岁的患者,差异有统计学意义(P<0.05)。(2)ALT异常率随HCV-RNA阳性率的增高而增加,并与HCV-RNA含量成正相关(r=0.999,P<0.05)。但ALT的水平与HCV-RNA含量无相关性(r=0.800,P>0.05)。(3)HCV-RNA阳性多伴有抗-HCV阳性(r=0.320),二者有很好的相关性。结论ELISA法检测抗-HCV在丙型肝炎诊断中仍旧为首选检测方法。联合应用HCV-RNA和抗-HCV及ALT检测,是诊断丙型肝炎最为可靠的方法。

Objective To study the application value of hepatitis C virus RNA detection (PCR-fluorescent probe) and hepatitis C virus antibody detection (ELISA, anti-HCV) in the diagnosis of hepatitis C. Methods The HCV-RNA and anti-HCV tests of 265 suspected HCV patients were reviewed, statistics of anti-HCV and HCV-RNA detection results and ALT level in different ages and gender was collected and analysized. Results ①Anti-HCV and HCV-RNA positive rates in men were higher than those in women, but there was no statistically significant difference (P>0.05).② Anti-HCV and HCV-RNA positive rates in＞40 years old patients were obviously higher than those in ≤40 years old patients ,with statistically significant difference (P<0.05); ALT abnormal rates also had significant difference between＞40 and ≤40 years old patients (P<0.05).③ ALT abnormal rate increased with increasing HCV-RNA positive rate and have positive correlation with HCV-RNA content(r=0.999,P<0.05). But the ALT levels did not correlate with HCV-RNA content (r=0.800,P>0.05). ④The abnormal rate of Anti-HCV was closely correlated with HCV-RNA (r=0.320); Anti-HCV, HCV-RNA and ALT had no statistically significant difference (P>0.05). Conclusions ①Anti-HCV and HCV-RNA detection have no relation with gender, but they have certain relevance with age. ②ELISA for detecting anti-HCV is still first choice in HCV. Combined use of HCV-RNA,anti-HCV and ALT detection, will be the most reliable and valuable method in diagnosis of HCV.