摘要
目的研究旋毛虫感染对过敏性哮喘小鼠血清TIgE、IL-4、IL-5的影响。方法随机将20只BALA/c雌性小鼠分为4组,每组5只,Ⅰ组为空白对照组,Ⅱ组为单纯过敏性哮喘组,Ⅲ组为感染旋毛虫后哮喘组,Ⅳ组为单纯感染旋毛虫组。首先,将200～300条旋毛虫囊包幼虫经口感染Ⅲ、Ⅳ组小鼠,建立旋毛虫感染模型。28d后,用卵清白蛋白分别对Ⅱ组和Ⅲ组小鼠进行致敏激发,建立过敏性哮喘模型。56d后,取小鼠血清,用ELISA法检测TIgE、IL-4、IL-5水平。结果4组小鼠总IgE水平分别为:(61.79±25.79)ng/ml、(437.08±75.68)ng/ml、(251.64±107.27)ng/ml和(446.12±74.32)ng/ml;IL-4水平分别为:(136.49±31.17)pg/L、(209.80±21.57)pg/L、(162.79±17.69)pg/L和(182.71±12.08)pg/L;IL-5水平分别为:(7.31±1.86)ng/L、(16.00±2.28)ng/L、(9.24±1.73)ng/L和(11.41±2.11)ng/L;与Ⅱ组相比较,Ⅲ组血清中总IgE、IL-4及IL-5水平明显降低(P<0.05)。结论旋毛虫感染可以抑制过敏性哮喘小鼠血清TIgE、IL-4及IL-5的表达。
Abstract
Objective To study the effect of Trichinella spiralis infection on total IgE, IL-4 and IL-5 in serum of the mice with allergic asthma. Methods Twenty female BALB/c mice were randomly divided into four groups of five mice each, including Ⅰ as control group, Ⅱ as allergic asthma group, Ⅲ as asthma followed by Trichinella spiralis infection, Ⅳ as Trichinella spiralis infected group. Firstly, the animals in group Ⅲ and Ⅳ were infected with 200-300 Trichinella spiralis muscle larvae. Twenty-eight days later, ovalbumin (OVA) was used to induce the allergic asthma for mice in group Ⅱ and Ⅲ, in order to establish asthma animal model. After 56 days, all mice were sacrificed, levels of TIgE, IL-4 and IL-5 in serum were measured by ELISA. Results The TIgE levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were (61.79±25.79) ng/ml,(437.08±75.68) ng/ml, (251.64±107.27) ng/ml and (446.12±74.32)ng/ml, respectively. The IL-4 levels in groupⅠ,Ⅱ, Ⅲ and Ⅳ were (136.49±31.17) pg/L, (209.80±21.57) pg/L, (162.79±17.69) pg/L and (182.71±12.08)pg/L, respectively. IL-5 levels in groupⅠ,Ⅱ, Ⅲ and Ⅳ were (7.31±1.86) ng/L, (16.00±2.29) ng/L,(9.24±1.73) ng/L and (11.41±2.11)ng/L, respectively. Compared with group Ⅱ, the TIgE、IL-4 and IL-5 levels in group Ⅲ decreased (P<0.05). Conclusions Trichinella spiralis infection can inhibit expression of the TIgE, IL-4 and IL-5 in serum of mice with allergic asthma
关键词
旋毛虫 /
过敏性哮喘 /
总IgE /
细胞因子
Key words
Trichinella spiralis /
allergic asthma /
TIgE /
cytokines
马萍,闫玉文,包春雨.
旋毛虫感染对过敏性哮喘小鼠血清总IgE、IL-4、IL-5的影响[J]. 武警医学. 2013, 24(6): 502-504
MA Ping,YAN Yuwen,and BAO Chunyu..
Experimental study on changes of serum total IgE, IL-4 and IL-5 in mice with Trichinella spiralis induced allergic asthma[J]. Medical Journal of the Chinese People Armed Police Forces. 2013, 24(6): 502-504
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