目的检测IL-23/IL-17轴在鼻息肉中的表达水平，探讨IL-23/IL-17轴在鼻息肉发病中的作用。方法收集在鼻内镜下慢性鼻-鼻窦炎鼻息肉手术患者鼻息肉组织(鼻息肉组)45例，因鼻中隔偏曲行下鼻甲部分切除术患者(对照组)下鼻甲黏膜组织30例，分别采用免疫组织化学方法和RT-PCR方法检测两组IL-23和IL-17的表达情况，并对两组IL-23和IL-17表达水平之间的关系进行研究。结果RT-PCR显示，鼻息肉组IL-23p19mRNA和IL-17mRNA表达水平明显高于对照组[(0.676±0.077)vs(0.212±0.015);(0.681±0.044)vs(0.220±0.044)]，两组比较差异均具有统计学意义(P＜0.01);免疫组化显示，鼻息肉组可见大量IL-23和IL-17阳性炎性浸润细胞，单位目标面积光密度均值明显高于对照组[(0.321±0.023)vs(0.199±0.011);(0.315±0.023)vs(0.211±0.029)]，两组比较差异均有统计学意义(均为P＜0.01);IL-17表达水平与IL-23表达水平呈正相关关系(基因表达水平r＝0.723，P<0.01;蛋白表达水平r=0.991，P<0.01)。结论IL-23、IL-17可能参与了慢性鼻-鼻窦炎鼻息肉的发病过程，并且IL-23可能通过调解鼻腔局部IL-17水平而发挥作用。
Abstract
Objective To investigate the expression of interleukin-23(IL-23) and interleukin-17(IL-17) axis in the nasal polyp patients and explore the role of IL-23/IL-17 axis in the nasal polyp. Method Forty-five nasal polyp(NP) patients’s NP tissue and 30 simple deviation of nasal septum patients’ normal inferior turbinate tissue were collected. mRNA and protein expression of IL-23 and IL-17 were detected by immunohistochemistry and RT-PCR. SPSS 17.0 software package was used for statistical analysis. test and Spearman coefficient were used to analyse and compare the expression level and the correlation.Results RT-PCR demomstrated that the average expression level of IL-23p19mRNA and IL-17mRNA in NP tissues [(0.676±0.077),(0.681±0.044)] had significant differences (P<0.01) from those in normal inferior turbinate tissues [(0.212±0.015), (0.220±0.044)]. Immunohistochemical staining demomstrated that there were much more IL-23 and IL-17 positive cells in NP patients than in the normal controls.The average expression levels of IL-23 and IL-17 protein in NP tissues [(0.321±0.023), (0.315±0.023)] were significantly different (P<0.01) from those in normal inferior turbinate tissues [(0.199±0.011), (0.211±0.029)]. IL-17mRNA positively correlated with IL-23p19mRNA(r=0.723,P<0.01),and IL-17 protein positively correlated with IL-23 protein(r=0.991,P<0.01). Conclusion IL-23 and IL-17 may involved in the chronic inflammation of NP.IL-23 may be play a role through adjusting the level of IL-17.
关键词
变应性鼻炎 /
鉴别诊断 /
治疗
Key words
interleukin-23 /
interleukin-17 /
chronic rhinosinustitis /
nasal polyp /
immunohistochemistry /
RT-PCR
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基金
国家自然科学基金(30872849);辽宁省自然科学基金(2011404013)
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