目的 观察脑瘫患者骨髓采集量与间充质干细胞(human mesenchymal stem cells，hMSCs)体外增殖时间的关系。方法 离心贴壁法分离脑瘫患者骨髓hMSCs，根据年龄分为幼儿组和儿童组;根据骨髓采集量分为小于20 ml(10~20 ml)组，大于21 ml(21~33ml)组，观察骨髓采集量与细胞传代时间的关系。结果 (1)幼儿及儿童组不同骨髓量P0、P1、P2、P3、P4代细胞传代时间无明显差异(P>0.05);(2)幼儿组和儿童组比较，hMSCs在P0、P1、P2、P3传代时间无统计学差异，而P4传代时间幼儿组快于儿童组(P<0.05)。结论 脑瘫患者体外培养hMSCs的体外增殖速度不随骨髓采集量的增加而增加。

Objective To study the effect of bone marrow collected from 30 patients with cerebral palsy amount on proliferation of bone marrow mesenchymal stem cells (human mesenchymal stem cells, hMSCs) isolated in vitro. Methods Wall attached hMSCs in bone marrow of patients with cerebral palsy were isolated by centrifugalization, cultured in vitro, the adherent cells were passaged and reserved. The patiants were divided, according to age, into infant group (1-3 years, n=14) and children group (3-12 years old, n=16); and were grouped, according to the amount of bone marrow harvested, infant group (10-20 ml, 6 cases) and more than 20 ml (20-33 ml, 8 cases). The relationship botween bone marrow cell amount harvested and passage time in the two groups of cerebral palsy pationts was observed. Results (1) In infant group and children group of patients with cerebral palsy, between the bone marrow amount and P0, P1, P2, P3, P4 generation cell passage time, there were no significant differences. (2) In the infant groups and children groups, there was no difference between passage time less than 20 ml group P3, where as P4 passage time performance in the infant group was significantly faster than that in the children group (t=3.15, P<0.02). (3) In infants and children greater than 20 ml groups, P0, P1, P2, P3 passage time had no significant difference (P>0.05), where as P4 passage time in the infant group was significantly faster than that in children group (t=2.23, P<0.05). Conclusions In patients with cerebral palsy, hMSCs in vitro proliferation rate is not increased with increasing amount of bone marrow harvested.