目的 研究宫颈癌细胞中HPV E6蛋白调控miRNA-145表达的分子机制及其在宫颈癌侵袭转移中的意义。方法 Northern-blot方法检测宫颈癌细胞中过表达p53对miR-145表达的影响;检测宫颈癌细胞中敲低HPV E6表达对miR-145表达的影响。Western-blot方法检测过表达HPV E6对内源性及外源性p53表达的影响;检测宫颈癌细胞中敲低HPV E6对外源性p53的表达的影响。应用划痕愈合实验，检测过表达miR-145的HeLa细胞与母本HeLa在细胞迁徙能力上的差异;Trans-well试验观察过表达miR-145的HeLa细胞与母本HeLa在细胞侵袭能力上的差异。结果 宫颈癌细胞中过表达p53可显著上调miR-145的表达;过表达HPV E6可降低p53的表达;宫颈癌细胞中敲低HPV E6表达可增强p53的表达，并且可增强双氧水诱导的miR-145表达;过表达miR-145抑制细胞的迁徙、侵袭能力。结论 HPV E6通过抑制p53下调miR-145表达，从而促进宫颈癌细胞侵袭。

Objective To study the molecular mechanisms of cervical cancer HPV infection regulating host cells miRNAs’ expression and its significance in invasion and metastasis of cervical cancer. Methods Northern blotting was used to detect the impact of over-expression of p53 on the expression of miR-145 in cervical carcinoma cells, compared with the impact of lowering HPV E6 on miR-145 expression. Western blotting was used to detect the impact of over-expression of the E6 on endogenous and exogenous p53 expression and influence of knockdown of HPV E6 cervical cancer cells on exogenous p53 expression. Scratch test was used to detect the different expressions of miR-145 in HeLa cells and the female parent HeLa cells in cell migration ability. Trans-well test was used to observe differences in the over-expression of miR-145 in HeLa cells and the female parent HeLa cell invasion. Results In cervical cancer cells, over-expression of p53 significantly increases expression of miR-145; over-expression of HPV E6 reduces the expression of p53 in cervical cancer cells; knockdown of HPV E6 expression in cervical cancer cells enhances the expression of exogenous p53 and the expression of miR-145 induced by hydrogen peroxide; over-expression of miR-145 inhibits cell migration and invasion of cervical cancer cells. Conclusions HPV E6 promotes the invasion and metastasis of cervical cancer cells, by inhibiting P53 and lowered the expression of miR-145.