目的 研究富血小板血浆(platelet-rich plasma，PRP)对于骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSC)增殖及矿化能力的影响。方法 改良Appel法采集兔PRP，同时培养该兔BMSC，将培养成功的BMSC分为实验组及对照组，实验组采用含10%PRP的培养液，对照组采用常规培养液，分别对两组细胞进行细胞增殖的检测(MTT法)、ATP活性检测(试剂盒)及矿化能力检测(Von-Kossa染色)。收集结果并进行统计。结果 实验组兔BMSC胞体丰满，细胞突起明显增长;实验组细胞增殖能力强于对照组(P＜0.01);实验组细胞在第4、8、12天时ALP活性强于对照组(P＜0.05)，第16天时无显著差别;实验组每孔矿化结节数(4.083±2.022)个，对照组每孔矿化结节数(3.417±1.285)个，两组比较无统计学差异。结论 PRP对BMSC的生长增殖有促进作用，但对该细胞的矿化能力无明显促进作用。

Objective To investigate the effect of platelet-rich plasma (PRP) on the proliferation and mineralization of bone marrow mesenchymal stem cells (BM-MSCs), thereby elucidating the possible application of PRP in bone tissue engineering. Methods Rabbit bone marrow aspirates were procured by modified Appel’s method. BM-MSCs were isolated and cultured in traditional medium with or without supplement of 10% PRP. The cell proliferation was assayed by MTT, cell osteogenic differentiation was assayed by using ATP activity examination kit and von-Kossa staining. Results The BM-MSCs cultured in PRP-supplemented medium were more spindle-like in morphology and had a significantly shorter doubling time in comparison with the control traditional culture without PRP supplement (P<0.01). The cells also displayed a higher ATP activity than control on days 4, 8, 12, and 16 (P<0.05). No statistically significant difference was exhibited at day 20 post-induction in ATP activity, and von-Kossa staining. Conclusions PRP enhances the proliferation of BM-MSCs and improves their ATP activity but has no effect on mineralization.